Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti
Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti
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DOI：
10.1111/j.1365-2583.2010.01032.x
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发表时间：
2010-12-01
影响因子：
2.6
通讯作者：
James, A. A.
中科院分区：
文献类型：
--
作者：
Mathur, G.;Sanchez-Vargas, I.;James, A. A.
受控的性别、阶段和组织特异性的抗病原体效应分子的表达对于控制蚊媒疾病的基因工程策略非常重要。成年女性唾液腺参与了病原体向宿主的传播，也是抗病原体效应分子表达的靶点。埃及伊蚊30Ka和30Kb基因仅在成年女性唾液腺中表达，并从263个核苷酸相隔的起始点不同地转录。这两个基因的5‘端和3’端的非翻译区足以在女性唾液腺的远侧叶同时表达两种不同的转基因产物。抗登革热效应基因膜NO蛋白(MNP)由30Kb启动子驱动，表达来自登革病毒2型基因组膜前蛋白编码区的反向重复RNA，显著降低蚊子唾液腺和唾液中病毒感染的患病率和平均强度。
Controlled sex-, stage- and tissue-specific expression of antipathogen effector molecules is important for genetic engineering strategies to control mosquito-borne diseases. Adult female salivary glands are involved in pathogen transmission to human hosts and are target sites for expression of antipathogen effector molecules. The Aedes aegypti 30K a and 30K b genes are expressed exclusively in adult female salivary glands and are transcribed divergently from start sites separated by 263 nucleotides. The intergenic, 5'- and 3'-end untranslated regions of both genes are sufficient to express simultaneously two different transgene products in the distal-lateral lobes of the female salivary glands. An antidengue effector gene, membranes no protein (Mnp), driven by the 30K b promoter, expresses an inverted-repeat RNA with sequences derived from the premembrane protein-encoding region of the dengue virus serotype 2 genome and reduces significantly the prevalence and mean intensities of viral infection in mosquito salivary glands and saliva.