Cutting edge: electronic counting of white blood cells.
Cutting edge: electronic counting of white blood cells.
复制标题
最前沿：白细胞电子计数。
DOI：
10.1039/b917138j
复制
发表时间：
2009
期刊：
影响因子：
6.1
通讯作者：
Irimia,Daniel
中科院分区：
文献类型：
--
作者：
Irimia,Daniel
几十年来，医生办公室里最常见的检查之一就是血细胞计数(CBC)。虽然这项测试简单且相对便宜，但对于各种情况来说，它是一种非常有效的筛查工具。例如，较低的红细胞数量可能是贫血的一个指标，进一步的研究可能会确定可能的原因。中性粒细胞数量较多可能表明感染正在进行中，而淋巴细胞数量的变化可能表明一些代谢性或增殖性疾病。CBC的起源与19世纪中叶发现的化学细胞斑点有关，通常在显微镜下在血细胞计数玻片上手动进行计数。到20世纪中叶，跨孔电阻抗的自动测量被引入来计算红细胞和白细胞，以及红细胞分数内的大小分布。目前，患者的血液样本大多使用基于荧光或光散射测量原理的自动流式细胞仪进行检测，不仅能够计算红细胞和白细胞，还能够计算不同的白细胞亚群。
For decades, one of the most commonly ordered tests in the doctor’s office has been the cell blood count (CBC). Although simple and relatively inexpensive, this test is a very effective screening tool for a wide range of conditions. For example, a lower number of red blood cells could be an indicator for anemia and further investigations could identify the likely cause. A higher number of neutrophils could indicate an infection in progress, while changes in the number of lymphocytes could reveal some metabolic or proliferative diseases. The origins of CBC are linked to the discovery of chemical cell stains in the middle of the 19th century and the count was usually done manually, under the microscope, on hemocytometer slides. By the middle of the 20th century, automated measurements of electric impedance across apertures were introduced to count red and white blood cells, and size distribution within the red blood cell fraction. Currently, blood samples from patients are examined mostly using automated flow cytometers, based on principles of fluorescence or light scattering measurements, and are capable of counting not only red and white blood cells, but also the different leukocyte subsets.