Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins
Influence of matrix solution conditions on the MALDI-MS analysis of peptides and proteins
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DOI：
10.1021/ac9507956
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发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Chait, BT
中科院分区：
文献类型：
--
作者：
Cohen, SL;Chait, BT
样品-基质制备程序极大地影响了肽和蛋白质的基质辅助激光解吸/电离（MALDI）质谱的质量，特别是当基质4-羟基-α-氰基肉桂酸用于分析肽和蛋白质的复杂混合物时，观察到显著的质量区分效应。的歧视效果被发现是强烈依赖于样品基质溶液的组成，pH值，和样品基质共晶生长的速率。这些研究结果表明，在执行和解释生物样品的MALDI分析时需要非常小心，结果还表明，在样品基质制备程序中存在反相色谱样尺寸，可以利用该尺寸来优化分析。目前的工作描述的条件下，肽和蛋白质的复杂混合物的大部分成分可以成功地测量。
Sample-matrix preparation procedures are shown to greatly influence the quality of the matrix-assisted laser desorption/ionization (MALDI) mass spectra of peptides and proteins, In particular, dramatic mass discrimination effects are observed when the matrix 4-hydroxy-alpha-cyanocinnamic acid is used for analyzing complex mixtures of peptides and proteins. The discrimination effects are found to be strongly dependent on the sample-matrix solution composition, pH, and the rates at which the sample-matrix cocrystals are grown. These findings demonstrate the need to exercise great care in performing and interpreting the MALDI analysis of biological samples, The results also indicate that there is a reverse-phase chromatographic-like dimension in the sample-matrix preparation procedures that can be exploited to optimize the analysis. The present work describes the conditions under which the majority of components of a complex mixture of peptides and proteins can be successfully measured.