beta-Tricalcium phosphate in the early phase of socket healing: an experimental study in the dog.
beta-Tricalcium phosphate in the early phase of socket healing: an experimental study in the dog.
复制标题
β-磷酸三钙在牙槽窝愈合早期的作用：对狗的实验研究。
DOI：
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发表时间：
2010
影响因子：
4.3
通讯作者：
J. Lindhe
中科院分区：
文献类型：
--
作者：
M. Araújo;B. Liljenberg;J. Lindhe
目标
本实验的目的是分析在拔牙和移植后的愈合过程中，宿主组织中加入β-磷酸三钙颗粒的过程。
材料和方法
使用了五只比格犬。切开上颌前磨牙4颗(3颗P(3)，2颗P(2))，拔除远中牙根，在新鲜拔牙槽内填充三聚氰酸钙。拔牙和移植手术的安排是这样的，即1天和3天的活组织检查，以及1周、2周和4周的愈合。测定细胞、纤维、血管、白细胞、矿化骨等组织元素。在脱蜡切片中，通过使用标记来鉴定表达抗转氨酸酸性磷酸酶、碱性磷酸酶和骨桥蛋白的结构和细胞。
结果
磷酸三钙颗粒的孔洞最初被红细胞填充，随后被矿化的骨取代。部分移植物材料被间充质细胞和炎性细胞侵入并解体。因此，肉芽组织和临时基质中出现了膜结合的小颗粒。在硬组织形成过程中，部分矿化(改性)的磷酸三钙颗粒被编织骨脊包围。
结论
研究表明，植入β-磷酸三钙的拔牙槽的早期愈合包括：(I)凝固物的形成，(Ii)被肉芽组织取代，以及(Iii)编织骨形成的临时基质。在这一过程中，生物材料显然也参与其中。
OBJECTIVES
The aim of this experiment was to analyze processes involved in the incorporation of beta-tricalcium phospate (TCP) particles in host tissue during healing following tooth extraction and grafting.
MATERIAL AND METHODS
Five beagle dogs were used. Four premolars in the maxilla ((3)P(3), (2)P(2)) were hemi-sected, the distal roots were removed and the fresh extraction socket filled with TCP. The tooth extraction and grafting procedures were scheduled in such a way that biopsies representing 1 and 3 days, as well as 1, 2, and 4 weeks of healing could be obtained. Tissue elements such as cells, fibers, vessels, leukocytes and mineralized bone were determined. In deparaffinized sections structures and cells that expressed Tratarate resistant acid phosphate, alkaline phosphatase, and osteopontin were identified by the use of markers.
RESULTS
The porosities of the TCP particles were initially filled with erythrocytes that subsequently were replaced with mineralized bone. Some of the graft material was invaded by mesenchymal and inflammatory cells and disintegrated. Thus, small membrane bound granules appeared in the granulation tissue and the provisional matrix. In the process of hard tissue formation, partly mineralized (modified) TCP particles became surrounded by ridges of woven bone.
CONCLUSIONS
It was demonstrated that the early healing of an extraction socket that had been grafted with beta-TCP involved (i) the formation of a coagulum that was (ii) replaced with granulation tissue and a provisional matrix in which (iii) woven bone could form. In this process the biomaterial was apparently involved.
影响因子：
2.9
作者：
BUTLER, WT
通讯作者：
BUTLER, WT
影响因子：
3
作者：
G. Pinero;M. Farach‐Carson;R. Devoll;J. Aubin;J. Brunn;W. Butler
通讯作者：
G. Pinero;M. Farach‐Carson;R. Devoll;J. Aubin;J. Brunn;W. Butler