Use of the elevated plus-maze test with opaque or transparent walls in the detection of mouse strain differences and the anxiolytic effects of diazepam
Use of the elevated plus-maze test with opaque or transparent walls in the detection of mouse strain differences and the anxiolytic effects of diazepam
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DOI：
10.1097/01.fbp.0000189811.77049.3e
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发表时间：
2006-02-01
影响因子：
1.6
通讯作者：
Yee, Benjamin K.
中科院分区：
文献类型：
--
作者：
Hagenbuch, Niels;Feldon, Joram;Yee, Benjamin K.
高架正迷宫是一种在啮齿动物中广泛使用的行为测试方法，用于检测抗焦虑药物和致焦虑药物。然而，在不同的实验室中，使用透明和不透明墙壁的仪器往往不同。当比较结果时，这种不一致可能代表文献中不必要的混淆。在这里，我们直接从(1)小鼠品系差异(C57BL/6,129/Sv和C3H/He)和(2)对安定(0.5，1.0和1.5 mg/kg，腹腔注射)的敏感性方面对两种设计进行了对比。两个迷宫的结果相似，表明129/Sv小鼠比C57BL/6或C3H小鼠更焦虑，透明的高架正迷宫倾向于鼓励所有三个品系的开放手臂探索。接下来，我们在两个迷宫中检查了安定对129/Sv菌株的影响。全身性安定1.5 mg/kg可增加在两个高架正迷宫中张开双臂的时间百分比；当剂量为0.5 mg/kg时，该药对两个高架正迷宫均无效。虽然我们的结果显示两个迷宫在检测焦虑相关行为的能力方面几乎没有实际差异，但两个高架加号迷宫在张开手臂探索方面的基线差异表明，透明设计可能更适合于检测焦虑性操作，而不透明迷宫可能更适合于检测焦虑操作。
The elevated plus-maze is a widely employed behavioural assay for anxiolytic and anxiogenic agents in rodents. Among laboratories, however, the apparatus often differs between the use of transparent and opaque walls. This inconsistency may represent an unnecessary confound in the literature when comparing results. Here, we directly contrasted the two designs with respect to (1) mouse strain differences (C57BL/6,129/Sv, and C3H/He) and (2) sensitivity to diazepam (0.5, 1.0, and 1.5 mg/kg, intraperitoneal). Both mazes yielded similar results indicating that 129/Sv mice were more anxious than C57BL/6 or C3H mice, with the transparent elevated plus-maze tending to encourage open-arm exploration in all three strains. Next, we-examined the effect of diazepam in the 129/Sv strain across the two mazes. Systemic diazepam at 1.5 mg/kg led to increased percentage time spent in the open arms in both elevated plus-mazes; the drug was ineffective in both elevated plus-mazes at a dose of 0.5 mg/kg. Although our results revealed little practical difference between the two mazes in terms of their ability to detect differences in anxiety-related behaviour, the baseline difference in open-arm exploration between the two elevated plus-mazes suggests that the transparent design may be more congenial for the detection of anxiogenic manipulations, and the opaque maze for anxiolytic manipulations.