Antimicrobial activity of cotton fabrics containing immobilized enzymes

Antimicrobial activity of cotton fabrics containing immobilized enzymes
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DOI:
10.1002/app.25821
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发表时间:
2007-05
影响因子:
3
通讯作者:
N. Ibrahim;M. Gouda;A. El-Shafei;O. Abdel-Fatah
N. Ibrahim;M. Gouda;A. El-Shafei;O. Abdel-Fatah
中科院分区:
化学3区
文献类型:
--
作者:
N. Ibrahim;M. Gouda;A. El-Shafei;O. Abdel-Fatah

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研究了α-淀粉酶、碱性果胶酶和漆酶在酯交联和铜螯合棉织物上的固定化。研究了影响固定化酶的载酶量和保留活力的因素。研究了获得更高固定程度和更好保留活性的适当条件。沿着。处理后的织物样品对革兰氏阴性和革兰氏阳性细菌,丝状和非丝状真菌的抗菌活性的程度进行了评价。抗微生物活性取决于底物的类型,即,铜螯合、酯交联和活化的棉基质,以及固定化酶的性质,即,碱性果胶酶> α-淀粉酶>漆酶。经处理的织物的抗微生物活性在洗涤至少十个连续洗涤循环后完全保持。进一步的连续洗涤循环,即,20或30个周期,对保留的抗菌功效具有实际负面影响。© 2007 Wiley Periodicals,Inc.应用聚合物科学杂志104:1754-1761,2007
Immobilization of α-amylase, alkaline pectinase, and laccase enzymes onto ester-crosslinked as well as Cu-chelated cotton fabrics were carried out. Factors affecting the extent of enzyme-loading and retention activities of immobilized enzymes were studied. Proper conditions for attaining higher extent of fixation along with better retained activity were studied. The degree of antimicrobial activity of treated fabric samples against gram-negative and gram-positive bacteria, filamentous, and nonfilamentous fungi were evaluated. The antimicrobial activity is determined by the type of substrate, i.e., Cu-chelated > ester-crosslinked and activated cotton substrate, and the nature of immobilized enzyme, i.e., alkaline pectinase > α-amylase > laccase, irrespective of the used microorganism. The antimicrobial activities of the treated fabrics are completely maintained after laundering at least ten consecutive wash cycles. Further consecutive wash cycles, i.e., 20 or 30 cycles, has practically negative impact on the retained antimicrobial efficacy. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 1754–1761, 2007