MODE OF BINDING OF QUERCETIN TO DNA
MODE OF BINDING OF QUERCETIN TO DNA
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DOI：
10.1093/mutage/9.3.193
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发表时间：
1994-05-01
期刊：
影响因子：
2.7
通讯作者：
HADI, SM
中科院分区：
文献类型：
--
作者：
AHMED, MS;RAMESH, V;HADI, SM
槲皮素-DNA复合物与槲皮素单独的差谱的特征在于在395 mm处的峰。随着离子强度的增加，可以看到差谱的幅度增加。槲皮素的吸光度和荧光光谱的变化表明，溴化乙锭能够取代槲皮素从槲皮素-DNA复合物。这些结果表明，槲皮素与DNA的结合不涉及静电相互作用，但可能是嵌入的性质。使用DNA酶I足迹技术的实验表明，类黄酮在DNA中不具有任何优选的结合位点。槲皮素-Cu（II）系统在DNA中的链断裂给出了核苷酸间键的大致均匀的切割模式。这导致观察到槲皮素-Cu（II）切割反应具有用作优选的DNA足迹试剂的潜力。
The difference spectrum of the quercetin-DNA, complex versus quercetin alone was characterized by a peak at 395 mm. An increase in the magnitude of difference spectrum was seen with increased ionic strength. Spectrophotometric changes in absorbance and fluorescence of quercetin showed that ethidium bromide is able to displace quercetin from the quercetin-DNA complex. These results indicate that the binding of quercetin to DNA does not involve electrostatic interactions but may be intercalative in nature. Experiments using DNase I footprinting technique showed that the flavonoid does not possess any preferred sites of binding in DNA. Strand scission in DNA by the quercetin-Cu(II) system gave a generally uniform cutting pattern of internucleotide bonds. This led to the observation that the quercetin-Cu(II) cleavage reaction has the potential of being used as preferred DNA footprinting reagent.