Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization
Hepatitis C virus NS5B protein is a membrane-associated phosphoprotein with a predominantly perinuclear localization
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DOI：
10.1006/viro.1996.8357
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发表时间：
1997-01-20
期刊：
影响因子：
3.7
通讯作者：
Lai, MMC
中科院分区：
文献类型：
--
作者：
Hwang, SB;Park, KJ;Lai, MMC
丙型肝炎病毒NS5B蛋白是一种依赖RNA的RNA聚合酶。为了研究该蛋白的性质和功能，我们在昆虫和哺乳动物细胞中表达了NS5B蛋白。发现NS5B在细胞质中以细小斑点存在，特别是集中在核周区域，这表明其与核膜、内质网或高尔基复合体相关。这一结论得到了NS5B与细胞膜相关的生物化学证明的支持。此外，还表明NS5B蛋白是磷蛋白。这些性质可能与其作为RNA聚合酶的功能有关。(C)1997学术出版社
Hepatitis C virus NS5B protein is an RNA-dependent RNA polymerase. To investigate the properties and function of this protein, we have expressed the NS5B protein in insect and mammalian cells. NS5B was found to be present as fine speckles in the cytoplasm, particularly concentrated in the perinuclear region, suggesting its association with the nuclear membrane, the endoplasmic reticulum, or the Golgi complex. This conclusion was supported by the biochemical demonstration that NS5B was associated with the membranes in the cells. Furthermore, it was shown that NS5B protein is a phosphoprotein. These properties may be related to its function as an RNA polymerase. (C) 1997 Academic Press