Androgens in male rat reproductive tract fluids: hypophysectomy and steroid replacement.
Androgens in male rat reproductive tract fluids: hypophysectomy and steroid replacement.
复制标题
雄性大鼠生殖道液体中的雄激素：垂体切除术和类固醇替代。
DOI：
10.1152/ajpendo.1985.248.3.e274
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Zegeye,B
中科院分区：
文献类型：
--
作者：
Turner,TT;Ewing,LL;Jones,CE;Howards,SS;Zegeye,B
男性生殖道腔内液体中雄激素浓度的控制尚不清楚。本实验旨在确定垂体切除术、垂体切除术加睾酮 (T) 和垂体切除术加孕烯醇酮治疗对成年大鼠睾丸和附睾管腔内雄激素浓度的影响。测定附睾血管、间质和管腔内的 T 和 5 α-二氢睾酮 (DHT) 浓度。还在光学显微镜下检查睾丸和附睾形态。至少 5 天的垂体切除术显着降低了血清、组织和生殖道腔内液体中 T 和 DHT 的浓度。 T 替代 14 天，产生的外周 T 浓度为 5 ng/ml，但不支持与对照相同的生精小管管腔内雄激素浓度；然而，睾丸网雄激素浓度与对照相似。孕烯醇酮以 2 mg X 大鼠-1 X 天-1 给药 14 天，并没有维持与对照相同的生精小管中的精子发生和管腔内 T 浓度；然而，当通过睾酮和孕烯醇酮治疗将管腔内和组织雄激素浓度维持在对照的10-20%时，精子发生的水平仍然较低。
The control of androgen concentrations in the intraluminal fluids of the male reproductive tract is not well understood. The present experiments were performed to determine the effects of hypophysectomy, hypophysectomy plus testosterone (T), and hypophysectomy plus pregnenolone treatment on intraluminal androgen concentrations in the adult rat testis and epididymis. T and 5 alpha-dihydrotestosterone (DHT) concentrations were determined in the vascular, interstitial, and intraluminal compartments of the epididymis. Testicular and epididymal morphology also were examined under light microscopy. Hypophysectomy of at least 5 days duration significantly reduced T and DHT concentrations in serum, tissues, and intraluminal fluids of the reproductive tract. T replacement for 14 days, which produced peripheral T concentrations of 5 ng/ml, did not support intraluminal androgen concentrations in the seminiferous tubules equivalent to controls; rete testis androgen concentrations were similar to controls, however. Pregnenolone administration at 2 mg X rat-1 X day-1 for 14 days did not maintain spermatogenesis nor intraluminal T concentrations in the seminiferous tubules equivalent to controls; however, a low level of spermatogenesis continued when intraluminal and tissue androgen concentrations were maintained at 10-20% of controls by either the testosterone and pregnenolone treatments.