Effects of Auraptene on IGF-1 Stimulated Cell Cycle Progression in the Human Breast Cancer Cell Line, MCF-7.
Effects of Auraptene on IGF-1 Stimulated Cell Cycle Progression in the Human Breast Cancer Cell Line, MCF-7.
复制标题
DOI：
10.1155/2012/502092
复制
发表时间：
2012
影响因子：
1.9
通讯作者：
Kleiner-Hancock H
中科院分区：
文献类型：
--
作者：
Krishnan P;Kleiner-Hancock H
人们正在研究Auraptene在皮肤癌、结肠癌、前列腺癌和乳腺癌等多种癌症模型中的化学预防作用。对于极光烯的化学预防作用，人们提出了许多作用机制，包括抗炎、抗增殖和抗凋亡作用。我们之前已经在N-甲基亚硝脲诱导的乳腺癌模型中表明，饮食中的Auapene(500ppm)显著延迟了肿瘤的潜伏期。肿瘤发生的时间延迟与肿瘤中细胞周期蛋白D1的表达显著降低相对应。由于细胞周期蛋白D1是细胞周期的主要调控因子，我们进一步研究了极光烯对MCF-7细胞周期及细胞周期相关基因的影响。在这里，我们发现极光显着抑制胰岛素样生长因子-1刺激的细胞周期的S期在MCF-7细胞，并显着改变许多基因的转录参与细胞周期。
Auraptene is being investigated for its chemopreventive effects in many models of cancer including skin, colon, prostate, and breast. Many mechanisms of action including anti-inflammatory, antiproliferative, and antiapoptotic effects are being suggested for the chemopreventive properties of auraptene. We have previously shown in the N-methylnitrosourea induced mammary carcinogenesis model that dietary auraptene (500 ppm) significantly delayed tumor latency. The delay in time to tumor corresponded with a significant reduction in cyclin D1 protein expression in the tumors. Since cyclin D1 is a major regulator of cell cycle, we further studied the effects of auraptene on cell cycle and the genes related to cell cycle in MCF-7 cells. Here we show that auraptene significantly inhibited IGF-1 stimulated S phase of cell cycle in MCF-7 cells and significantly changed the transcription of many genes involved in cell cycle.