Ionic liquid 1-ethyl-3-methylimidazolium acetate: an attractive solvent for native chemical ligation of peptides
Ionic liquid 1-ethyl-3-methylimidazolium acetate: an attractive solvent for native chemical ligation of peptides
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DOI：
10.1016/j.tetlet.2014.04.102
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发表时间：
2014-07-02
影响因子：
1.8
通讯作者：
Imhof, Diana
中科院分区：
文献类型：
--
作者：
Kuehl, Toni;Chen, Ming;Imhof, Diana
离子液体乙酸 1-乙基-3-甲基咪唑鎓 ([C(2)mim][OAc]) 成功用作肽片段天然化学连接的替代溶剂，以产生模型肽 LYRAXCRANK（X = G、A、L、N、Q、K 和 F）。常用的含有硫醇添加剂（如苯硫酚、苄硫醇）的缓冲体系可以用无毒的离子液体[C(2)mim][OAc]替代。除了提高肽在 [C(2)mim][OAc] 中的溶解度之外，还发现连接反应的产率和速率得到有效提高。 (C) 2014 Elsevier Ltd. 保留所有权利。
The ionic liquid 1-ethyl-3-methylimidazolium acetate ([C(2)mim][OAc]) was successfully used as alternative solvent for native chemical ligation of peptide fragments to produce model peptide LYRAXCRANK (X = G, A, L, N, Q, K, and F). The commonly used buffer system including thiol additives such as thiophenol and benzyl mercaptan can be replaced by the nontoxic ionic liquid [C(2)mim][OAc]. In addition to improving the solubility of the peptides in [C(2)mim][OAc], yields and rates of the ligation reactions were found to be efficiently enhanced. (C) 2014 Elsevier Ltd. All rights reserved.