Identification and phylogenetic analysis of pathogen-Ewardsiela tarda from cultured turbot(Scophthalmus maximus)
Identification and phylogenetic analysis of pathogen-Ewardsiela tarda from cultured turbot(Scophthalmus maximus)
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养殖大菱鲆病原菌Ewardsiela tarda的鉴定及系统发育分析
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
H. Jie
中科院分区：
文献类型：
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作者：
H. Jie
细菌菌株1101菌株是从患病大菱鲆中分离到的一株从山东半岛某养殖场养殖的大菱鲆（Scophthalmus maximus）中分离到1101菌株，经攻毒试验证明该菌株具有较高的毒力，采用16 S rRNA序列测定、生理生化特性鉴定和系统发育分析等方法对菌株1101进行鉴定，结果表明，菌株1101为革兰氏阴性、氧化酶阴性、过氧化氢酶阳性。经API 20 E鉴定，菌株1101为迟缓爱德华氏菌（Ewardsiela tarda），其可靠性为99%;对1101菌株的1419 bp DNA进行测序，并与GenBank中的其他细菌进行同源性分析和系统发育分析，结果表明，该菌株与迟缓爱德华氏菌（Ewardsiela tarda）的相似性最高，为99%;根据该菌株的形态特征、生理生物学特性和16 S rDNA同源性比较，药敏试验表明，1101菌株对多粘菌素敏感，而其他药物对该菌株无明显抑制作用.
A strain of bacteria(strain number 1101) was isolated from diseased turbot(Scophthalmus maximus)in a fish farm in Shandong peninsula.Challenge test proved that the strain had higher virulence.Identifying methods including 16S rRNA sequence test,physiological and biochemical characteristics and phylogenetic analysis were employed to identify the strain 1101 respectively.The results show that strain1101 was Gram negative,oxidase negative,catalase positive. Result by API20E indicated that strain 1101 was Ewardsiela tarda with 99% reliability.A 1419 bp DNA was sequenced and compared with other bacteria in GenBank.Homology analysis and phylogenetic study showed that it has the highest similarity to Ewardsiela tarda with 99% similarity.According to morphological features,physiological and biological characteristics and 16S rDNA homology comparison of the bacteria,the pathogenic bacteria were Ewardsiela tarda.Drugs sensitivity tests showed that strain1101 has sensitive to polymyxin.While other drugs had no obvious inhibitory activity to the strain.