Delivery of MicroRNA-let-7c-5p by Biodegradable Silica Nanoparticles Suppresses Human Cervical Carcinoma Cell Proliferation and Migration
Delivery of MicroRNA-let-7c-5p by Biodegradable Silica Nanoparticles Suppresses Human Cervical Carcinoma Cell Proliferation and Migration
复制标题
通过可生物降解的二氧化硅纳米颗粒递送 MicroRNA-let-7c-5p 可抑制人宫颈癌细胞的增殖和迁移。
DOI：
10.1166/jbn.2020.2989
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发表时间：
2020-11-01
影响因子：
2.9
通讯作者：
Li, Y. J.
中科院分区：
文献类型：
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作者：
Shao, L. Y.;Wang, R. R.;Li, Y. J.
人类宫颈癌是最常见的妇科恶性肿瘤。纳米技术的不断发展使得纳米材料在癌症治疗中得到广泛应用。纳米粒子因其表面效应和小尺寸效应而可作为基因载体。 MicroRNA-let-7c-5p (miR-let-7c-5p) 属于 let-7 家族。尽管有报道称miR-let-7c-5p在多种癌症中发挥肿瘤抑制作用，但miR-let-7c-5p在宫颈癌进展中的确切作用和机制尚不清楚。在这项研究中，我们合成了具有高 pDNA/siRNA 负载率的花形 SiO2 -PEI 纳米颗粒。这种带有 miR-let-7c-5p 表达质粒的纳米颗粒可以有效地将 miR-let-7c-5p 转移到人上皮癌细胞 (HeLa) 细胞中。此外，纳米材料与基因治疗的结合可以在极低细胞毒性的条件下抑制癌症的发展。这些发现提供了基于F-SiO2-聚乙烯亚胺/miR-let-7c-5p（FSP-let-7c-5p）纳米颗粒的新抗癌策略，并表明miR-let-7c-5p/IGF-1R/PI3K/AKT和-catenin/SLUG可以作为治疗宫颈癌的新的潜在靶点。
Human cervical cancer is the most common gynecological malignancy. The continuous development of nanotechnology has allowed the wide use of nanomaterials in cancer treatment. Nanoparticles can be used as gene carriers because of their surface effect and small-size effect. MicroRNA-let-7c-5p (miR-let-7c-5p) belongs to the let-7 family. Although it has been reported to exert a tumor suppressive effect in a variety of cancers, the exact role and mechanism of miR-let-7c-5p in the progression of cervical cancer are unclear. In this study, we synthesized flower-shaped SiO₂ -PEI nanoparticles with high pDNA/siRNA loading rates. This nanoparticle with miR-let-7c-5p-expressed plasmid could effectively transfer miR-let-7c-5p to human epithelial carcinoma (HeLa) cells. In addition, the combination of nanomaterials and gene therapy could inhibit the development of cancer under the conditions of extremely low cytotoxicity. These findings provided a new anticancer strategy based on F-SiO₂ -polyethyleneimine/miR-let-7c-5p (FSP-let-7c-5p)nanoparticles and indicated that miR-let-7c-5p/IGF-1R/PI3K/AKT and -catenin/SLUG could be used as new potential targets for the treatment of cervical cancer.