A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal
A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal
复制标题
一种新型铜绿假单胞菌菌株同时进行异养硝化-好氧反硝化和好氧除磷
DOI：
10.1016/j.watres.2022.118823
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发表时间：
2022-07-09
期刊：
影响因子：
12.8
通讯作者：
Chen, Si
中科院分区：
文献类型：
--
作者：
Huang, Mei-Qi;Cui, You -Wei;Chen, Si
废水中氮和磷酸盐的去除依赖于不同的功能细菌。本研究通过梯度稀释和异养硝化-好氧反硝化-好氧除磷（HNADPR）技术从活性污泥中分离出一株与铜绿假单胞菌有关的新型菌株。在C/N比为10、P/N比为0.1、温度为30℃、pH为7.5 ~ 8.5的条件下，菌株对氨氮的去除率为87%，对磷酸盐的去除率为97%。修正的Gompertz模型可以很好地拟合异养铵硝化、好氧亚硝酸盐/硝酸盐反硝化和好氧除磷工艺。功能基因扩增表明，氨脱除遵循完整的HN-AD途径(NH4+ -> NH2OH -> NO2- -> NO3？-> no2 -> no -> n20 -> n-2)。磷酸盐的去除只在好氧条件下发生，在厌氧条件下停止。在连续的有氧循环中，菌株持续吸收磷酸盐。在废水中，磷酸盐被好氧转化为细胞膜、细胞内和细胞外的聚合物底物（EPS）。磷以磷酸单酯的形式聚集在EPS中。提出了菌株snpr -01的有氧除磷模型，以提高我们对HNADPR新细菌功能的理解。
Nitrogen and phosphate removal from wastewater relies on different functional bacteria. In this study, a novel strain affiliated with Pseudomonas aeruginosa was isolated from activated sludge by gradient dilution and per-formed heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal (HNADPR). The strain showed an ammonium removal efficiency of 87% and a phosphate removal efficiency of 97% under optimal conditions, such as C/N ratio of 10, P/N ratio of 0.1, temperature of 30C, and pH of 7.5-8.5. The modified Gompertz model could fit well the heterotrophic ammonium nitrification, aerobic nitrite/nitrate denitrification, and aerobic phosphate removal processes. Functional gene amplification indicated that ammonium removal followed the complete HN-AD pathway (NH4+ -> NH2OH -> NO2- -> NO3? -> NO2- -> NO -> N2O -> N-2). Phosphate removal only occurred under aerobic conditions and ceased under anaerobic conditions. In successive aerobic cycles, the strain persistently took up phosphate. In wastewater, phosphate was aerobically converted into cell membrane, intracellular and extracellular polymeric substrates (EPS). Phosphorus in the form of phosphate monoester was pooled in EPS. A hypothetic aerobic phosphate removal model for strain SNDPR-01 is proposed to improve our understanding of the novel bacterial function of HNADPR.