Transplantation of mesenchymal stem cells within a poly(lactide-co-ε-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model
Transplantation of mesenchymal stem cells within a poly(lactide-co-ε-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model
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DOI：
10.1093/eurjhf/hfn017
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发表时间：
2009-02-01
影响因子：
18.2
通讯作者：
Kim, Kyung-Soo
中科院分区：
文献类型：
--
作者：
Jin, Jiyong;Jeong, Sung In;Kim, Kyung-Soo
心脏组织工程已被提议作为修复心肌梗塞（MI）的合适方法。有证据表明，具有支架组合的细胞比仅细胞植入物更有效。然而，迄今为止，还没有关于弹性可生物降解聚（丙交酯-共-ε-己内酯）（PLCL）支架的研究。本研究的目的是探讨间充质干细胞 (MSC) 与弹性可生物降解 PLCL 支架移植在大鼠 MI 模型中的作用。通过冷冻损伤方法诱导 MI 十天后，将盐水对照、MSC、PLCL 支架或接种 MSC 的 PLCL 支架移植到心脏上。移植后四周，评估心脏功能和组织学。移植的间充质干细胞在受损区域存活并分化为心肌细胞。 MSC+PLCL组左心室射血分数较生理盐水组增加23%； MSC 组也较高。 MSC+PLCL组梗塞面积较生理盐水组减少29%；间充质干细胞加PLCL应该是心脏组织工程的绝佳组合。
Cardiac tissue engineering has been proposed as an appropriate method to repair myocardial infarction (MI). Evidence suggests that a cell with scaffold combination was more effective than a cell-only implant. Nevertheless, to date, there has been no research into elastic biodegradable poly(lactide-co-epsilon-caprolactone) (PLCL) scaffolds. The aim of this study was to investigate the effect of mesenchymal stem cells (MSCs) with elastic biodegradable PLCL scaffold transplants in a rat MI model.Ten days after inducing MI through the cryoinjury method, a saline control, MSC, PLCL scaffold, or MSC-seeded PLCL scaffold was transplanted onto the hearts. Four weeks after transplantation, cardiac function and histology were evaluated. Transplanted MSCs survived and differentiated into cardiomyocytes in the injured region. Left ventricular ejection fraction in the MSC + PLCL group increased by 23% compared with that in the saline group; it was also higher in the MSC group. The infarct area in the MSC + PLCL group was decreased by 29% compared with that in the saline group; it was also reduced in the MSC group.Mesenchymal stem cells plus PLCL should be an excellent combination for cardiac tissue engineering.