Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae
Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae
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酿酒酵母细胞周期依赖性胸苷酸合酶基因的转录调控
DOI：
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发表时间：
1988
影响因子：
5.3
通讯作者：
R. Storms
中科院分区：
文献类型：
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作者：
E. McIntosh;R. Ord;R. Storms
我们之前已经表明，从酵母TMP1基因表达的转录物水平在酵母细胞周期中周期性波动。然而，尚不清楚周期性表达是由转录水平上的调节机制引起的，还是由细胞周期阶段依赖性TMP1转录物稳定性变化水平上的调节机制引起的。在这篇报告中，我们现在表明TMP1转录物的周期性表达主要是由转录起始位点上游的序列在转录水平上控制的。我们还将周期性转录所需的上游序列定位到一个150碱基对的区域，并表明该区域编码一个具有周期性上游激活序列特性的元件。本研究定义的调控区域显然不包含与报道的细胞周期调控的HO内切酶或酿酒酵母的组蛋白H2A和H2B基因相似的一致序列。
We have previously shown that transcript levels expressed from the yeast TMP1 gene fluctuate periodically during the yeast cell cycle. However, it was not known whether periodic expression resulted from a regulatory mechanism acting at the level of transcription or a regulatory mechanism acting at the level of cell cycle stage-dependent changes in the stability of the TMP1 transcript. In this report we now show that the periodic expression of TMP1 transcript is primarily controlled at the level of its transcription by sequences which are upstream of its transcription initiation sites. We also localized the upstream sequences necessary for periodic transcription to a 150-base-pair region and show that this region encodes an element(s) with the properties of a periodic upstream activating sequence. The regulatory region defined in this study apparently does not contain consensus sequences similar to those reported for the cell cycle-regulated HO endonuclease or for the histone H2A and H2B genes of Saccharomyces cerevisiae.
酵母中的调节蛋白。
影响因子：
11.1
作者：
Guarente,L
通讯作者：
Guarente,L