Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production
Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production
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DOI：
10.1039/c1ee02112e
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发表时间：
2011-10-01
影响因子：
32.5
通讯作者：
Wyman, Charles E.
中科院分区：
文献类型：
--
作者：
DeMartini, Jaclyn D.;Pattathil, Sivakumar;Wyman, Charles E.
为了更好地了解水热预处理如何降低植物细胞壁的顽抗性，我们应用了高通量方法（“糖组分析”），使用一套全面的植物聚糖定向单克隆抗体来监测杨属生物量的结构/可提取性变化。使用同一工具包中选定的抗体通过免疫标记验证糖组分析研究的结果。这些研究中使用的单克隆抗体阵列足够大，足以监测大多数植物细胞壁多糖中发生的变化。这些技术的结果证明了在预处理诱导的解构过程中植物细胞壁中发生的结构变化的顺序，即木质素-多糖相互作用的最初破坏与果胶和阿拉伯半乳聚糖的损失相一致；随后显着去除木聚糖和木葡聚糖。此外，这项研究还表明，木质素含量本身并不影响顽抗性。相反，木质素和多糖在细胞壁内的整合以及它们之间的相互联系发挥着更大的作用。
To better understand how hydrothermal pretreatment reduces plant cell wall recalcitrance, we applied a high throughput approach ("glycome profiling") using a comprehensive suite of plant glycan-directed monoclonal antibodies to monitor structural/extractability changes in Populus biomass. The results of glycome profiling studies were verified by immunolabeling using selected antibodies from the same toolkit. The array of monoclonal antibodies employed in these studies is large enough to monitor changes occurring in most plant cell wall polysaccharides. Results from these techniques demonstrate the sequence of structural changes that occur in plant cell walls during pretreatment-induced deconstruction, namely, the initial disruption of lignin-polysaccharide interactions in concert with a loss of pectins and arabinogalactans; this is followed by significant removal of xylans and xyloglucans. Additionally, this study also suggests that lignin content per se does not affect recalcitrance; instead, the integration of lignin and polysaccharides within cell walls, and their associations with one another, play a larger role.