Long-Term Effects of TCM Yangqing Kangxian Formula on Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulating Nuclear Factor-κB Signaling.
Long-Term Effects of TCM Yangqing Kangxian Formula on Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulating Nuclear Factor-κB Signaling.
复制标题
中药养清康纤方通过调节核因子-κB信号对博来霉素诱导的大鼠肺纤维化的长期影响
DOI：
10.1155/2017/2089027
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Li J
中科院分区：
文献类型：
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作者：
Li M;Li Y;Li J;Zhao P;Bai Y;Feng S;Liu X;Wang Y;Bian Q;Li J
目的评价养肾康口服液的临床疗效和远期疗效，并对其作用机制进行初步探讨。材料与方法IPF大鼠从第1天至第42天分别给予YKF、泼尼松或吡非尼酮，然后间隔28天至第70天不干预。分别于第7、14、28、42、70天测定用力肺活量、组织病理学、羟脯氨酸含量、肺系数、血炎性细胞数、肺组织炎性细胞因子水平及核因子-κB信号通路相关蛋白的表达。结果IPF大鼠肺组织HYP含量、Ashcroft评分、肺系数和肺纤维化血细胞数显著增加，FVC显著下降。从第7天到第70天，治疗组上述各项指标均有改善，尤以养肾方组改善更明显。从第7天到第70天，养肾方组肿瘤坏死因子-α的表达显著降低，而干扰素-γ的表达显著增加，胞浆核因子-kappaB激酶抑制物β(IKK-β)、核因子kappaB抑制物α(IκBα)和核因子-κB的磷酸化明显下调。结论养肺口服液通过抗炎、抗纤维化作用，对肺纤维化具有良好的保护和远期作用。
Objective We aimed to evaluate the therapeutic effects and long-term effects of YKF and dissect the potential mechanisms. Materials and Methods IPF rats were given YKF, prednisone, or pirfenidone, respectively, from day 1 to day 42, followed by a 28-day nonintervention interval through day 70. Forced vital capacity (FVC), histopathology, hydroxyproline (HYP) contents, lung coefficient, blood inflammatory cell populations, inflammatory cytokine levels of the lung tissues, and the expression of proteins involved in nuclear factor- (NF-) κB signaling pathway were evaluated on days 7, 14, 28, 42, and 70. Results HYP contents, Ashcroft scores, lung coefficient, and pulmonary fibrosis blood cell populations increased significantly in IPF rats, while FVC declined. All the above-mentioned parameters were improved in treatment groups from day 7 up to day 70, especially in YKF group. The mRNA and protein expressions of tumor necrosis factor- (TNF-) α significantly decreased, while interferon- (IFN-) γ significantly increased, and phosphorylations of cytoplasm inhibitor of nuclear factor kappa-B kinase β (IKKβ), inhibitor of nuclear factor kappa-B α (IκBα), and NF-κB were obviously downregulated in YKF group from day 7 to day 70. Conclusion YKF has beneficial protective and long-term effects on pulmonary fibrosis by anti-inflammatory response and alleviating fibrosis.