Stiffness-Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles
Stiffness-Dependent In Vitro Uptake and Lysosomal Acidification of Colloidal Particles
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DOI：
10.1002/anie.201409693
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发表时间：
2015-01-19
影响因子：
16.6
通讯作者：
Parak, Wolfgang J.
中科院分区：
文献类型：
--
作者：
Hartmann, Raimo;Weidenbach, Marcel;Parak, Wolfgang J.
胶体颗粒的物理化学性质决定了它们被细胞吸收。对于一系列的微粒，只有一个参数，机械刚度，是不同的，而其他参数，如大小，形状和电荷保持不变。通过分析单个颗粒轨迹（包括内吞作用的进展）原位监测摄取，内吞作用源自每个颗粒周围的局部pH测量。证据表明，软颗粒与低刚度运输速度比刚性的溶酶体。
The physico-chemical properties of colloidal particles determine their uptake into cells. For a series of microparticles only one parameter, the mechanical stiffness, was varied, whereas other parameters such as size, shape, and charge were kept constant. The uptake was monitored in situ by analyzing individual particle trajectories including the progress of endocytosis, derived from local pH measurements around each particle. Evidence is presented that soft particles with low stiffness are transported faster to lysosomes than stiffer ones.