REGIONAL DIFFERENCES IN THE EFFECTS OF CHRONIC ETHANOL ADMINISTRATION ON [H-3] ZOLPIDEM FINDING IN RAT-BRAIN
REGIONAL DIFFERENCES IN THE EFFECTS OF CHRONIC ETHANOL ADMINISTRATION ON [H-3] ZOLPIDEM FINDING IN RAT-BRAIN
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DOI：
10.1111/j.1530-0277.1995.tb00966.x
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发表时间：
1995-08-01
影响因子：
3.2
通讯作者：
DUNCAN, GE
中科院分区：
文献类型：
--
作者：
DEVAUD, LL;MORROW, AL;DUNCAN, GE
已经观察到[H-3]唑吡坦结合与特定脑区α(1)、β(2)和伽马(2)亚基的伽马氨基丁酸(GABA(A))受体mRNA的存在有很强的关联。这与观察到的这些相同区域的单个神经元对唑吡坦和乙醇的敏感性有关。以前使用匀浆结合方法的研究表明，慢性酒精暴露后，[H-3]唑吡坦结合水平发生了微小的变化。这项研究是为了确定慢性乙醇给药对[H-3]唑吡坦结合水平的影响是否具有地区性特异性。慢性乙醇注射引起下丘、黑质和内侧隔[H-3]唑吡坦(5 NM)结合的微小但显著的变化。[H-3]唑吡坦在下丘和黑质的结合量增加，在内侧隔减少。在被分析的任何其他大脑区域，包括大脑皮层和小脑，都没有发现[H-3]唑吡坦结合的显著差异。这些发现表明，慢性乙醇给药对[H-3]唑吡坦结合的影响很小，尽管它们是以位置特异性和双向方式发生的。此外，[H-3]唑吡坦结合的变化与GABA(A)受体亚单位表达的变化之间没有相关性。
A strong association has been observed between [H-3]zolpidem binding and the presence of gamma-aminobutyric acid (GABA(A)) receptor mRNA for alpha(1)-, beta(2)-, and gamma(2)-subunits in specific brain regions. This correlates with observed sensitivity of individual neurons to zolpidem and ethanol in these same regions. Previous studies using homogenate binding approaches showed small alterations in [H-3]zolpidem binding levels after chronic ethanol exposure. This study was undertaken to ascertain if there is regional specificity of the effects of chronic ethanol administration on [H-3]zolpidem binding levels. Chronic ethanol administration induced small, but significant alterations in [H-3]zolpidem (5 nM) binding in the inferior colliculus, substantia nigra, and the medial septum. [H-3]Zolpidem binding was increased in the inferior colliculus and substantia nigra, and decreased in the medial septum. No significant differences in [H-3]zolpidem binding were noted in any other brain area analyzed, including the cortex and cerebellum. These findings show that chronic ethanol administration has small effects on [H-3]zolpidem binding, although they occur in a site-specific and bidirectional manner. Moreover, there is no correlation between changes in [H-3]zolpidem binding and alterations in GABA(A) receptor subunit expression.