Multi-frequency ultrasound-assisted dialysis modulates the self-assembly of alcohol-free zein-sodium caseinate to encapsulate curcumin and fabricate composite nanoparticles
Multi-frequency ultrasound-assisted dialysis modulates the self-assembly of alcohol-free zein-sodium caseinate to encapsulate curcumin and fabricate composite nanoparticles
复制标题
多频超声辅助透析调节无醇玉米醇溶蛋白-酪蛋白酸钠自组装封装姜黄素并制备复合纳米颗粒
DOI：
10.1016/j.foodhyd.2021.107110
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发表时间：
2021-08-19
影响因子：
10.7
通讯作者：
Yu, Xiaojie
中科院分区：
文献类型：
--
作者：
Chen, Huiling;Xu, Baoguo;Yu, Xiaojie
开发多种玉米蛋白分布机制来封装疏水营养品已成为食品工业的热点。本研究采用多频超声辅助透析法制备稳定的纳米颗粒。超声工作模式和酪蛋白酸钠调节了纳米粒子体系的物理化学性质、分子相互作用和微观结构。当超声频率为20/40 kHz时，玉米蛋白酪氨酸钠的粒径、zeta电位和PDI分别为225.94 +/- 3.84 nm、- 29.97 +/- 1.49 mv和0.19 +/- 0.08。此外，超声和酪蛋白酸钠在提高纳米颗粒储存稳定性方面具有协同作用。荧光和CD结果表明，超声波通过改变蛋白的局部微环境和结构，促进酪蛋白酸钠与玉米蛋白的相互作用。FTIR结果表明，疏水相互作用、氢键相互作用和静电相互作用是杂化纳米颗粒形成的主要驱动力。超声辅助透析是一种有效的包封姜黄素的方法。XRD结果表明，姜黄素包封成功，20/40 kHz超声频率组合可使包封率提高90.19 +/- 0.33%，使姜黄素的热稳定性、贮存稳定性和抗氧化活性提高86.91 +/- 0.72%。原子力显微镜（AFM）和扫描电镜（SEM）显示复合纳米颗粒具有均匀、规则的球形结构。结果表明，多模超声辅助透析不仅可以包封疏水生物活性化合物，构建给药体系，而且在模拟胃肠道条件下具有控释作用，对疏水保健品具有较好的保护作用和理想的缓释效果。
Developing a number of zein distribution mechanisms that encapsulate hydrophobic nutraceuticals has become a hot topic in food industry. In this study, the multi-frequency ultrasound-assisted dialysis method was used to fabricate stable nanoparticles. The ultrasound working mode and sodium caseinate modulated physicochemical properties, molecular interactions and microstructure of the nanoparticles system. The optimal ultrasonic frequency of 20/40 kHz resulted in Zein-sodium caseinate particle size, zeta potential and PDI values of 225.94 +/- 3.84 nm, - 29.97 +/- 1.49 mv, and the 0.19 +/- 0.08, respectively. Additionally, ultrasound and Sodium Caseinate have a synergistic effect in improving the storage stability of nanoparticles. The results of fluorescence and CD demonstrated that ultrasound promoted the interaction between Sodium Caseinate and zein, by changing the local microenvironment and structure of the protein. FTIR results showed that the dominant driving force in the formation of hybrid nanoparticles was hydrophobic interaction, hydrogen bond and electrostatic interaction. Ultrasound-assisted dialysis was an effective strategy to encapsulate curcumin. XRD showed that curcumin was successfully encapsulated, and the 20/40 kHz ultrasound frequency combination improved encapsulation rate by 90.19 +/- 0.33% as well as thermal stability, storage stability and antioxidant activity of curcumin (86.91 +/- 0.72%). AFM and SEM showed uniform and regular spherical structure of composite nanoparticles. The findings revealed not only the multi-mode ultrasound assisted dialysis could be utilized to encapsulate hydrophobic bioactive compounds, which constructed delivery system, but also it provided a controlled release under simulated gastrointestinal conditions, and a better protection and desirable sustained-release of hydrophobic nutraceuticals.