The Effect of Electroporation Type Pulsed Electric Fields on DNA in Aqueous Solution
The Effect of Electroporation Type Pulsed Electric Fields on DNA in Aqueous Solution
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DOI：
10.1177/153303461000900412
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Rubinsky, Boris
中科院分区：
文献类型：
--
作者：
Golberg, Alex;Rubinsky, Boris
电穿孔是一种物理现象，其中施加在细胞上的脉冲电场产生细胞膜的瞬时（可逆）或永久（不可逆）透化。不可逆电穿孔是食品工业中的一种重要灭菌方法，也是医学上一种重要的微创组织消融技术。受最近在不可逆电穿孔细胞中观察到的细胞凋亡等标记染色的启发，我们研究了电穿孔类型电脉冲对溶液中裸DNA完整性的影响。使用凝胶电泳分析，我们表明，不可逆的电穿孔类型的脉冲有能力影响裸DNA在溶液中。结果发现，一些电参数，导致细胞死亡的不可逆电穿孔也引起变化的裸DNA暴露于相同的程序。我们的分析初步表明，一些电穿孔类型的电脉冲在DNA分子中引起切口。因此，不可逆电穿孔中细胞死亡的机制可能也包括对DNA的损伤。然而，这项工作并没有研究电穿孔诱导的电极腐蚀副产物，如铝离子对DNA完整性的可能影响，这也应该在未来研究。一般来说，由于电穿孔现象的应用广泛用于医学和生物技术，目前的研究表明，进一步研究电穿孔型电脉冲对DNA的影响是必要的。
Electroporation is a physical phenomenon in which pulsed electric fields applied across a cell produce transient (reversible) or permanent (irreversible) permeabilization of the cell membrane. Irreversible electroporation is an important method of sterilization in the food industry and it is becoming an important minimally invasive tissue ablation technique in medicine. Motivated by recent observations of apoptosis like marker stains in irreversibly electroporated cells we performed a study on the effects of electroporation type electric pulses on the integrity of naked DNA in solution. Using gel electrophoresis analyses we show that pulses of the irreversible electroporation type have the ability to affect the naked DNA in solution. It is found that some electric parameters that lead to cell death by irreversible electroporation also cause changes in the naked DNA exposed to the same procedure. Our analysis tentatively suggests that some electroporation type electric pulses cause nicks in the DNA molecule. Therefore, it is possible that the mechanisms of cell death in irreversible electroporation also include damages to the DNA. However, this work did not investigate the possible effects of electroporation induced electrode corrosion byproducts, such as Al3+ ions on DNA integrity; which should be also studied in the future. In general, since electroporation phenomena based applications are widely used in medicine and biotechnology, the current study suggests that further research into the effects of electroporation type electric pulses on the DNA are warranted.