Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins
Novel 13C direct detection experiments, including extension to the third dimension, to perform the complete assignment of proteins
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DOI：
10.1016/j.jmr.2005.08.011
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Pierattelli, R
中科院分区：
文献类型：
--
作者：
Bermel, W;Bertini, I;Pierattelli, R
由于探头技术的改进和新的核磁共振实验的发展，碳-13直接检测核磁共振方法是可行的。我们在这里提出了一套完整的实验，基于C'直接检测，开发执行蛋白质的主链和侧链（芳香环除外）的完整分配。这种策略为要求苛刻的情况（顺磁性和/或大分子）提供了替代解决方案，并且通常可与常规实验结合使用。(c)2005年爱思唯尔公司All rights reserved.
Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments. (c) 2005 Elsevier Inc. All rights reserved.