Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: An interlaboratory reproducibility study
Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: An interlaboratory reproducibility study
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DOI：
10.1016/j.ijcard.2016.07.214
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发表时间：
2016-11-01
影响因子：
3.5
通讯作者：
Henein, Michael Y.
中科院分区：
文献类型：
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作者：
Djekic, Demir;Pinto, Rui;Henein, Michael Y.
背景：最近，一种脂质组学方法能够识别扰动的脂肪酰链(FAC)和鞘脂部分，可以根据冠状动脉钙化的严重程度对患者进行分层，这是一种亚临床动脉粥样硬化的形式。然而，在推广应用之前，这些发现还没有被复制。本研究的目的是通过在钙化性冠状动脉疾病(CCAD)患者组中复制以往的脂肪组学研究结果来评估脂组学方法的重复性。方法：根据患者的钙化评分(CS)、无钙化(CS：0；n=26)、轻度钙化(CS：1-250；n=27)和重度钙化(CS：>；250；n=17)将患者分为以下组。每个患者采集两份血清样本，由两个不同的实验室在不同的国家和时间点进行对比分析，采用液-质联用非靶向脂组学方法。结果：两个实验室分别检测到6种相同的代谢物，区分了冠状动脉严重钙化患者和非钙化患者。此外，两种分析的相对强度显示出很高的相关系数。在疾病组血清中，18碳FAC的磷脂酰胆碱部分呈低强度，20：4的FAC呈高强度。此外，还检测到了3种常见的鞘磷脂蛋白。结论：这是第一次在CCAD患者中广泛应用脂类组学应用的实验室间重复性研究。CCAD患者的脂谱应用在高度专业化和经验丰富的实验室中具有非常高的重复性，并可以应用于临床实践，以避免患者的诊断辐射。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Background: Recently a lipidomics approach was able to identify perturbed fatty acyl chain (FAC) and sphingolipid moieties that could stratify patients according to the severity of coronary calcification, a form of subclinical atherosclerosis. Nevertheless, these findings have not yet been reproduced before generalising their application. The aim of this study was to evaluate the reproducibility of lipidomics approaches by replicating previous lipidomic findings in groups of patients with calcific coronary artery disease (CCAD).Methods: Patients were separated into the following groups based on their calcium score (CS); no calcification (CS: 0; n = 26), mild calcification (CS: 1-250; n = 27) and severe calcification (CS: >250; n = 17). Two serum samples were collected from each patient and used for comparative analyses by 2 different laboratories, in different countries and time points using liquid chromatography coupled to mass spectrometry untargeted lipidomics methods.Results: Six identical metabolites differentiated patients with severe coronary artery calcification from those with no calcification were found by both laboratories independently. Additionally, relative intensities from the two analyses demonstrated high correlation coefficients. Phosphatidylcholine moieties with 18-carbon FAC were identified in lower intensities and 20: 4 FAC in higher intensities in the serum of diseased group. Moreover, 3 common sphingomyelins were detected.Conclusion: This is the first interlaboratory reproducibility study utilising lipidomics applications in general and specifically in patients with CCAD. Lipid profiling applications in patients with CCAD are very reproducible in highly specialised and experienced laboratories and could be applied in clinical practice in order to spare patients diagnostic radiation. (C) 2016 Elsevier Ireland Ltd. All rights reserved.