Allergy development is associated with consumption of breastmilk with a reduced microbial richness in the first month of life
Allergy development is associated with consumption of breastmilk with a reduced microbial richness in the first month of life
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DOI：
10.1111/pai.13176
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发表时间：
2019-12-11
影响因子：
4.4
通讯作者：
Carmen Collado, Maria
中科院分区：
文献类型：
--
作者：
Dzidic, Majda;Mira, Alex;Carmen Collado, Maria
背景：早期不同微生物群的定植似乎对儿童时期适当的免疫成熟起着至关重要的作用。母乳微生物群是婴儿重要的微生物来源，与母体的IgA抗体一起转移。我们之前观察到，儿童时期的过敏发展与1个月大的婴儿对肠道微生物群的异常IgA反应有关，此时IgA抗体主要来自母乳喂养的婴儿。目的通过一项产前和产后补充罗伊氏乳杆菌的干预试验，了解母乳中微生物组成和iga包被菌与儿童过敏发生的关系。方法采用流式细胞分选和16S rRNA基因测序相结合的方法，对产后1个月采集的40例母亲的母乳进行IgA细菌识别，这些母亲的孩子在7岁前有或没有出现过敏和哮喘症状。结果给有过敏表现的儿童喂奶的细菌丰富度明显低于健康儿童喂奶的细菌丰富度。益生菌处理影响母乳微生物群组成。然而，在母乳中，健康儿童的母亲与过敏儿童的母亲之间，iga包被细菌的比例、细菌总负荷和iga包被模式是相似的。结论婴儿出生后第一个月食用微生物丰富度降低的母乳可能在儿童过敏发展中起重要作用。
Background Early colonization with a diverse microbiota seems to play a crucial role for appropriate immune maturation during childhood. Breastmilk microbiota is one important source of microbes for the infant, transferred together with maternal IgA antibodies. We previously observed that allergy development during childhood was associated with aberrant IgA responses to the gut microbiota already at 1 month of age, when the IgA antibodies are predominantly maternally derived in breastfed infants. Objective To determine the microbial composition and IgA-coated bacteria in breastmilk in relation to allergy development in children participating in an intervention trial with pre- and post-natal Lactobacillus reuteri supplementation. Methods A combination of flow cytometric cell sorting and 16S rRNA gene sequencing was used to characterize the bacterial recognition patterns by IgA in breastmilk samples collected one month post-partum from 40 mothers whose children did or did not develop allergic and asthmatic symptoms during the first 7 years of age. Results The milk fed to children developing allergic manifestations had significantly lower bacterial richness, when compared to the milk given to children that remained healthy. Probiotic treatment influenced the breastmilk microbiota composition. However, the proportions of IgA-coated bacteria, the total bacterial load and the patterns of IgA-coating were similar in breastmilk between mothers of healthy children and those developing allergies. Conclusion Consumption of breastmilk with a reduced microbial richness in the first month of life may play an important role in allergy development during childhood.