Identification and quantification of molecular species of diacyl glyceryl ether by reversed-phase high-performance liquid chromatography with refractive index detection and mass spectrometry.
Identification and quantification of molecular species of diacyl glyceryl ether by reversed-phase high-performance liquid chromatography with refractive index detection and mass spectrometry.
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通过反相高效液相色谱、折射率检测和质谱法对二酰基甘油醚的分子种类进行鉴定和定量。
DOI：
10.1016/s0021-9673(00)01240-1
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Fujimoto
中科院分区：
文献类型：
--
作者：
Y. Endo;Misako Tagiri;Hwan;K. Fujimoto
本文建立了一种利用配备折射率检测器和电喷雾电离和飞行时间质谱仪（LC-RI-MS）的高效液相色谱法（HPLC）鉴定和定量二酰基甘油醚（DAGE）分子种类的方法。采用十八烷基硅胶柱，以乙腈和二氯甲烷的混合物（65:35,v/v）为洗脱液进行高效液相色谱。当LC-RI-MS方法应用于合成DAGEs的混合物时；1- 16 -2,3-二油酰甘油（O-16:0-18:1-18:1）、1- 0 -十八烷基-2,3-二油酰甘油（O-18:0-18:1-18:1）、1- 0 -十八烷基-2,3-二油酰甘油（O-18:0-18:1-18:1）、1- 0 -十八烷基-2,3-二油酰六烯酰甘油（O-18:0-22:6-22:6）、1- 0 -十八烷基-2,3-二油酰六烯酰甘油（O-18:1-22:6-22:6），在折射率色谱上获得了良好的分离和定量。所有合成的DAGEs在质谱上都观察到一个伪分子离子[M+NH4]+和一个单酰基甘油醚离子[M−RCO2]+。这些质谱可以很容易地鉴别出DAGE中的脂肪酸和甘油醚。采用LC-RI-MS方法对星状叠合肌中提取的DAGEs进行分析，在LC-RI-MS图谱上发现了约18个峰，DAGEs的主要分子种类为O-16:0-18:1-18:1。
We have developed a method to identify and quantify the molecular species of diacyl glyceryl ether (DAGE) using high-performance liquid chromatography (HPLC) equipped with a refractive index detector and an electrospray ionization and time of flight mass spectrometer (LC–RI–MS). An octadecyl silica column with a mixture of acetonitrile and dichloromethane (65:35, v/v) as an eluant was used for the HPLC. When the LC–RI–MS method was applied to a mixture of synthetic DAGEs; 1-O-hexadecyl-2,3-dioleoylglycerol (O-16:0–18:1–18:1), 1-O-octadecyl-2,3-dioleoylglycerol (O-18:0–18:1–18:1), 1-O-octadecenyl-2,3-dioleoylglycerol (O-18:1–18:1–18:1), 1-O-octadecyl-2,3-didocodahexaenoylglycerol (O-18:0–22:6–22:6), and 1-O-octadecenyl-2,3-didocosahexaenoylglycerol (O-18:1–22:6–22:6), good separation and quantification were obtained on the refractive index chromatogram. A pseudo-molecular ion [M+NH4]+and a monoacyl glyceryl ether ion [M−RCO2]+were observed for all synthetic DAGEs on the mass spectrum. It was found that the fatty acids and glyceryl ether in DAGE could be easily identified by these mass spectra. When this LC–RI–MS method was applied to the DAGEs extracted from muscle of Stromateus stellatus, approximately 18 peaks were observed on LC–RI–MS chromatograms and the major molecular species of DAGEs were identified as O-16:0–18:1–18:1.