Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum
Identification of Leg Chemosensory Genes and Sensilla in the Apolygus lucorum
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光色姬鼠足化学感应基因和感器的鉴定
DOI：
10.3389/fphys.2020.00276
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发表时间：
2020-04-15
影响因子：
4
通讯作者：
Zhang, Yongjun
中科院分区：
文献类型：
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作者：
Li, Zibo;Zhang, Yaoyao;Zhang, Yongjun
绿盲蝽（半翅目：盲蝽科）是危害棉花等经济作物的主要害虫之一。众所周知，腿在昆虫的化学反应中起着重要的作用。本研究采用RNA转录组测序和qPCR方法，对绿盲蝽足部参与近距离或接触性化学交流的化学感觉蛋白进行了研究。对成虫前肢、中肢和后肢的转录组分析结果表明，在香蝽的腿中共鉴定出20个气味结合蛋白（OBP）基因、8个化学感觉蛋白（CSP）基因、1个气味受体（OR）基因、1个嗜离子受体（IR）基因和1个感觉神经元膜蛋白（SNMP）基因。与之前的天线转录组数据相比，5个csp、IR21a和SNMP2a在腿中被新发现。qPCR分析结果表明，这些化学感觉基因在昆虫的前腿、中腿和后腿中普遍表达。此外，通过扫描电镜观察，在盲蝽腿上发现了毛感器（长直型毛感器Str1、长弯型毛感器Str2）、毛感器（长1型毛感器Sch1、长2型毛感器Sch2、长3型毛感器Sch3）、基感器（中长型基感器Sba1、短型基感器Sba2）和Bohm刚毛（BB）等4种类型。此外，感受器在后腿上的数量最多，而前腿上的数量最少。我们的数据提供了有价值的见解，以了解黄斑蝽腿的化学接受。
Apolygus lucorum (Hemiptera: Miridae), one of the main insect pests, causes severe damage in cotton and many other economic crops. As is well-known, legs play important roles in the chemoreception of insects. In this study, the putative chemosensory proteins in legs of A. lucorum involved in close or contact chemical communication of adult bugs were investigated using RNA transcriptome sequencing and qPCR methods. Transcriptome data of forelegs, middle legs and hind legs of adult bugs demonstrated that 20 odorant binding protein (OBP) genes, eight chemosensory protein (CSP) genes, one odorant receptor (OR) gene, one ionotropic receptor (IR) gene and one sensory neuron membrane protein (SNMP) gene were identified in legs of A. lucorum. Compared to the previous antennae transcriptome data, five CSPs, IR21a and SNMP2a were newly identified in legs. Results of qPCR analysis indicated that all these putative chemosensory genes were ubiquitously expressed in forelegs, middle legs and hind legs of bugs. Furthermore, four types of sensilla on legs of A. lucorum including sensilla trichodea (subtypes: long straight sensilla trichodea, Str1; long curved sensilla trichodea, Str2), sensilla chaetica (subtypes: sensilla chaetica 1, Sch1; sensilla chaetica 2, Sch2; and sensilla chaetica 3, Sch3), sensilla basiconca (subtypes: medium-long sensilla basiconca, Sba1; short sensilla basiconca, Sba2) and Bohm bristles (BB) were found using scanning electron microscopy. Additionally, the largest number of sensilla was observed on hind legs, while the forelegs had the smallest number of sensilla. Our data provide valuable insights into understanding the chemoreception of legs in A. lucorum.