Critical interval of somal calcium transient after neurite transection determines B104 cell survival
Critical interval of somal calcium transient after neurite transection determines B104 cell survival
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DOI：
10.1002/jnr.20606
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发表时间：
2005-09-15
影响因子：
4.2
通讯作者：
Fishman, HM
中科院分区：
文献类型：
--
作者：
Nguyen, MP;Bittner, GD;Fishman, HM
轴突或树突切断后，神经细胞可能存活或死亡。神经突横断后，（< 50 μ m）负载Fura-2的B104神经母细胞瘤的细胞体（大鼠脑源性）细胞中，体钙浓度（SCC）经历三相瞬时变化：快速（横断后0-0.15分钟[PT]）上升期，随后是早期（0.15-1.5分钟PT）快速衰减阶段，并由后期（1.5-60分钟PT）缓慢衰减阶段，恢复SCC到损伤前水平。在第三瞬时相的关键间隔（1.5-12.5分钟PT）中的SCC与细胞命运相关，即，大多数排除染料（恢复屏障）并死亡的横切细胞在该临界间隔内具有比排除染料并在24小时PT负载BAPTA（体Ca 2+螯合）时存活的横切细胞显著更高（P < 0.005）的SCC，在该临界间隔之前而不是之后，与没有BAPTA负载的横切细胞相比，存活细胞的百分比增加。此外，大多数横断细胞死亡，尽管成功的屏障恢复表现出一致的特征与细胞凋亡启动期间的临界间隔的SCC，包括半胱天冬酶激活和质膜磷脂酰丝氨酸易位。这些数据表明，减少细胞存活的损伤附近的索马是由于钙离子启动的细胞凋亡在临界间隔的第三阶段的SCC瞬态。(c)2005 Wiley-Liss，Inc.
Nerve cells may survive or die after axohal or dendritic transection. After neurite transection near (< 50 mu m) the cell body of Fura-2-loaded B104 neuroblastoma (rat brain-derived) cells, the somal calcium concentration (SCC) undergoes a three-phase transient change: a rapid (0-0.15-min posttransection [PT]) rise phase, followed by an early (0.15-1.5-min PT) rapid decay phase, and succeeded by a late (1.5-60-min PT) slower decay phase that, restores SCC to preinjury levels. The SCC in a critical interval (1.5-12.5 min PT) of the third transient phase correlates with cell fate, i.e., most transected cells that exclude dye (restore a barrier) and die have a significantly higher (P < 0.005) SCC in this critical interval than do transected cells that exclude dye and survive at 24-hr PT Loading BAPTA (chelation of somal Ca2+) before, but not after, the critical interval increases the percentage of cells that survive compared to that of cells transected without BAPTA loading. Furthermore, most transected cells that die despite successful barrier restoration exhibit characteristics consistent with apoptosis initiated during the critical interval of the SCC, including caspase activation and plasmalemmal phosphaticlylserine translocation. These data suggest that decreased cell survival for injuries near the soma is due to Ca2+-initiated apoptosis during the critical interval of the third phase of the SCC transient. (c) 2005 Wiley-Liss, Inc.