Early events in natural resistance to bone marrow transplantation. Use of radiolabeled bone marrow cells.
Early events in natural resistance to bone marrow transplantation. Use of radiolabeled bone marrow cells.
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对骨髓移植的自然抵抗的早期事件。
DOI：
10.1097/00007890-198606000-00004
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发表时间：
1986
期刊：
影响因子：
6.2
通讯作者：
Kiesche,A
中科院分区：
文献类型：
--
作者：
Carlson,GA;Marshall,ST;Kiesche,A
对脾集落形成单位(CFU-S)增殖的天然抵抗力见于某些骨髓捐献者和受照射宿主的组合。为了研究骨髓移植后的早期事件，并确定基因决定的CFU-S抑制是由于移植细胞从脾中清除或抑制其增殖，我们在注射前用放射性I-脱氧尿苷标记新鲜分离的骨髓或长期骨髓培养中的增殖细胞。在新鲜分离的骨髓和外周血单个核细胞中都标记了不同种类的细胞混合物；标记的细胞中包括cfu-S前体细胞，这表明放射性标记的细胞群体中cfu-S的数量减少。
Natural resistance against the proliferation of splenic colony-forming units (CFU-S) is seen in certain combinations of bone marrow donors and irradiated hosts. In order to examine the early events following bone marrow transplantation and to determine whether genetically determined CFU-S repression is due to elimination of the transplanted cells from the spleen or to inhibition of their proliferation, we labeled proliferating cells in freshly isolated bone marrow or long-term bone marrow cultures (LTBMC) with radioactive I-iododeoxyuridine prior to injection. A heterogeneous mixture of cells was labeled in both freshly isolated marrow and LTBMC; CFU-S precursors were among the cells labeled as indicated by reduction of CFU-S numbers in the radiolabeled cell population.