Enhancing Biocompatibility without Compromising Material Properties: An Optimised NaOH Treatment for Electrospun Polycaprolactone Fibres
Enhancing Biocompatibility without Compromising Material Properties: An Optimised NaOH Treatment for Electrospun Polycaprolactone Fibres
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DOI：
10.1155/2019/4605092
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Cartmell, Sarah H.
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文献类型：
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作者：
Bosworth, Lucy A.;Hu, Wanxiao;Cartmell, Sarah H.
这项研究首次提出了电纺聚己内酯(PCL)纤维在氢氧化钠(NaOH)中浸泡的优化方案，以改善表面亲水性，从而在不影响材料性能的情况下实现生物兼容性。这项研究包括两个目标：(1)确定主要的NaOH浓度(0、0.1、1和10M)和浸泡时间(0、1、4和24小时)，以在对拉伸性能影响最小的情况下提高亲水性；(2)一旦确定，就进行材料表征和体外测试以进行验证。1M 4h(NaOH浓度：1M，浸泡时间：4h)改善了亲水性(0M NaOH和0h浸泡时间下排列的纤维从97+/-3度减少到6+/-2度；0M 0h时的随机纤维从105+/-4度减少到15+/-7度)，而对拉伸强度的影响最小(排列和随机的损失分别为9%和6%)。1M4h处理的支架材料性能没有明显变化，但显著改善了3T3成纤维细胞种植4h后的蛋白质吸附和附着、活力和伸长率。因此，1M 4h是电纺PCL成功湿化学处理的最佳方法，并且提供了一种简单、经济的方法，可以在不影响支架完整性的情况下容易地提高生物相容性。
This research presents the first optimised protocol for submersion of electrospun polycaprolactone (PCL) fibres in sodium hydroxide (NaOH) to improve surface hydrophilicity, and hence biocompatibility, without compromising material properties. The study comprised two aims: (1) identify the leading NaOH concentration (0, 0.1, 1, and 10M) and submersion time (0, 1, 4, and 24h) to improve hydrophilicity with minimal impact on tensile properties and (2) once identified, undertake material characterisation and in vitro testing for validation. 1M 4h (NaOH concentration: 1M, submersion time: 4h) improved hydrophilicity (aligned fibres at 0M NaOH and 0h submersion time reduced from 97 +/- 3 degrees to 6 +/- 2 degrees; and random fibres at 0M 0h reduced from 105 +/- 4 degrees to 15 +/- 7 degrees) with minimal impact on tensile strength (9% and 6% loss aligned and random, respectively). 1M 4h-treated scaffolds demonstrated no significant change in material properties, yet notably improved protein adsorption and attachment, viability and elongation of 3T3 fibroblasts 4h postseeding. Thus, 1M 4h is optimal for successful wet chemical treatment of electrospun PCL and presents a simple and economical method to easily enhance biocompatibility without compromising scaffold integrity.