TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes
TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes
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DOI：
10.1074/jbc.m009863200
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发表时间：
2001-04-13
影响因子：
4.8
通讯作者：
Woodman, P
中科院分区：
文献类型：
--
作者：
Bishop, N;Woodman, P
E类空泡蛋白分选(VPs)蛋白是酵母内吞途径中受体适当分选所必需的，很可能在多囊泡体的生物发生中发挥作用。我们已经确定Vps28p的哺乳动物同源蛋白是一个221个氨基酸的胞浆蛋白，它与TSG101/哺乳动物VPS23相互作用形成多蛋白复合体的一部分。免疫共沉淀和交联实验表明，hVPS28和TSG101直接相互作用，结合需要TSG101保守的C-末端部分的结构信息。TSG101和hVPS28以胞质为主。然而，当另一个E类VPs蛋白的显性-负性突变体-人Vps4表达诱导内体空泡化时，TSG101和hVPS28的一部分都转移到这些空泡的表面。我们得出结论，TSG101及其相互作用组分直接参与内体分选。
Class E vacuolar protein sorting (vps) proteins are required for appropriate sorting of receptors within the yeast endocytic pathway, and most probably function in the biogenesis of multivesicular bodies. We have identified the mammalian orthologue of Vps28p as a 221-amino acid cytosolic protein that interacts with TSG101/ mammalian VPS23 to form part of a multiprotein complex. Co-immunoprecipitation and cross-linking experiments demonstrated that hVPS28 and TSG101 interact directly and that binding requires structural information within the conserved C-terminal portion of TSG101. TSG101 and hVPS28 are predominantly cytosolic. However, when endosomal vacuolization was induced by the expression of a dominant-negative mutant of another class E vps protein, human VPS4, a portion of both TSG101 and hVPS28 translocated to the surface of these vacuoles. We conclude that TSG101 and its interacting components are directly involved in endosomal sorting.