Generation of superoxide by purified brain nitric oxide synthase.
Generation of superoxide by purified brain nitric oxide synthase.
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DOI：
10.1016/s0021-9258(18)35745-4
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发表时间：
1992-12
期刊：
影响因子：
--
通讯作者：
S. Pou;W. S. Pou;D. Bredt;S. Snyder;Gerald M. Rosen
中科院分区：
文献类型：
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作者：
S. Pou;W. S. Pou;D. Bredt;S. Snyder;Gerald M. Rosen
脑型一氧化氮合酶(NOS)利用NADPH和钙/钙调蛋白作为代谢L-精氨酸为一氧化氮(NO)和L-瓜氨酸的辅因子，含有黄素FAD和FMN的识别位点。使用自旋捕获技术和电子自旋共振光谱相结合，我们报告了大脑一氧化氮合酶产生超氧阴离子O2-。以钙/钙调蛋白依赖的方式。一氧化氮合酶的“特异性抑制剂”L和L对O2-有不同的影响。一代。对于L-NMMA，O2-。产生不受影响，而L的名字，抑制这种自由基是浓度依赖的。
Brain nitric oxide synthase (NOS), which utilizes NADPH and calcium/calmodulin as cofactors for metabolizing L-arginine to nitric oxide (NO) and L-citrulline, contains recognition sites for the flavins FAD and FMN. Using a spin-trapping technique combined with electron spin resonance spectroscopy, we report that brain NOS generates superoxide O2-. in a calcium/calmodulin-dependent manner. The “specific inhibitors” of NOS, NG-monomethyl L-arginine (L-NMMA), and NG-nitro-L-arginine methyl ester (L-NAME), have different effects on O2-. generation. For L-NMMA, O2-. production is unaffected, while for L-NAME, inhibition of this free radical is concentration-dependent.