GROWTH-RESPONSES OF COLIFORM BACTERIA TO RECOMBINANT BOVINE CYTOKINES
GROWTH-RESPONSES OF COLIFORM BACTERIA TO RECOMBINANT BOVINE CYTOKINES
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DOI：
10.3168/jds.s0022-0302(93)77425-1
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发表时间：
1993-04-01
影响因子：
3.5
通讯作者：
SORDILLO, LM
中科院分区：
文献类型：
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作者：
HOGAN, JS;TODHUNTER, DA;SORDILLO, LM
在体外测定了10株大肠杆菌对重组牛细胞因子的生长反应。从牛IMI中获得的6株大肠埃希菌和4株肺炎克雷伯菌分离株对重组牛白细胞介素-1 β、白细胞介素-2和干扰素-γ的生长反应进行了检测。以10（4）、10（3）、10（2）和10 U/ml培养基检测细胞因子。所用培养基为合成组织培养基、化学成分确定的合成细菌生长培养基和UHT灭菌牛奶。随着培养基中干扰素-γ浓度的增加，合成组织培养基和UHT牛奶中的细菌计数略有增加。重组牛干扰素-γ在对数生长期增加细菌种群，但不影响稳定生长期的细菌数量。在三种培养基中，细菌生长反应与白细胞介素-2或白细胞介素-1 β浓度无关。细胞因子的细菌生长反应与血清敏感性、干奶牛分泌物中分离株的生长、获得分离株的IMI持续时间或细菌种类的差异无关。
Growth responses of 10 coliform isolates to recombinant bovine cytokines were measured in vitro. Six Escherichia coli and four Klebsiella pneumoniae isolates obtained from bovine IMI were tested for growth responses to recombinant bovine interleukin-1beta, interleukin-2, and interferon-gamma. Cytokines were tested at 10(4), 10(3), 10(2), and 10 U/ml of media. Media used were a synthetic tissue culture medium, a chemically defined synthetic bacterial growth medium, and UHT sterilized milk. Bacterial counts in the synthetic tissue culture medium and UHT milk increased slightly as concentration of interferon-gamma in the media increased. Recombinant bovine interferon-gamma increased bacterial populations during the log growth phase but did not affect the number of bacteria in stationary growth phase. Bacterial growth responses were not related to either interleukin-2 or interleukin-1beta concentrations in any of the three media. Bacterial growth responses to cytokines were not related to differences in either serum susceptibility, growth of isolates in dry cow secretion, duration of IMI from which isolates were obtained, or bacterial species.