Diverse Helotiales associated with the roots of three species of Arctic Ericaceae provide no evidence for host specificity.
Diverse Helotiales associated with the roots of three species of Arctic Ericaceae provide no evidence for host specificity.
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DOI：
10.1111/j.1469-8137.2011.03703.x
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发表时间：
2011-07
期刊：
影响因子：
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通讯作者：
J. Walker;Laura Aldrich‐Wolfe;Amanda K. Riffel;Holly Barbare;Nicholas B. Simpson;Justin Trowbridge;
中科院分区：
文献类型：
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作者：
J. Walker;Laura Aldrich‐Wolfe;Amanda K. Riffel;Holly Barbare;Nicholas B. Simpson;Justin Trowbridge;
杜鹃花科菌根真菌利用氮源的能力不同，可能是维持杜鹃花科存在的生态系统中真菌和植物多样性的重要组成部分。在这项研究中，我们测试了三种共生杜鹃花科植物的真菌群落是否存在差异。通过培养依赖型和非培养型技术对北极苔原中的 Cassiope tetragona、Empetrum nigrum 和 Vaccinium vitis-idaea 根部定殖的真菌进行了表征。在实验室检测中测试了培养的真菌定植越橘的能力。纯培养的 Helotiales 分为 8 个支系，以 Phialocephala-Acephala 复合体为主。这些分支的代表，加上与 Irpex 属（多孔菌目）有亲和力的未知担子菌，在 V. uliginosum 细胞内定殖。通过直接PCR、克隆和测序检测到的Helotiales被分配到14个进化枝，并且以杜鹃花根霉复合体的成员为主。排序分析表明，培养依赖性和非培养检测提供了根部真菌群落的不同视图，但没有宿主特异性的证据。这些数据表明，杜鹃花的根部寄生着以 Helotiales 为主的多种真菌群落。然而，这些真菌群落不太可能受到真菌宿主偏好的控制。维持根共生群落高度多样性的机制仍有待阐明。
Ericoid mycorrhizal fungi differ in their abilities to use nitrogen sources and may be integral to maintaining fungal and plant diversity in ecosystems in which Ericaceae occur. In this study, we tested whether the fungal communities differ among three species of co-occurring Ericaceae. Fungi colonizing Cassiope tetragona, Empetrum nigrum and Vaccinium vitis-idaea roots in the Arctic tundra were characterized via culture-dependent and culture-independent techniques. The cultured fungi were tested for their ability to colonize Vaccinium uliginosum in laboratory-based assays. The pure-cultured Helotiales were grouped into eight clades and dominated by the Phialocephala-Acephala complex. Representatives of these clades, plus an unknown basidiomycete with affinity to the genus Irpex (Polyporales), colonized V. uliginosum intracellularly. The Helotiales detected by direct PCR, cloning and sequencing were assigned to 14 clades and dominated by members of the Rhizoscyphus ericae complex. Ordination analyses indicated that culture-dependent and culture-independent assays provided distinct views of root fungal communities, but no evidence for host specificity. These data suggest that ericaceous roots host diverse fungal communities dominated by the Helotiales. However, these fungal communities are unlikely to be controlled by fungal host preferences. The mechanisms maintaining high diversity in root-symbiotic communities remain to be elucidated.