Molecular analysis of highly pathogenic avian influenza virus of subtype H5N1 isolated from wild birds and mammals in northern Germany
Molecular analysis of highly pathogenic avian influenza virus of subtype H5N1 isolated from wild birds and mammals in northern Germany
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DOI：
10.1099/vir.0.82300-0
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Mundt, Egbert
中科院分区：
文献类型：
--
作者：
Weber, Siegfried;Harder, Timm;Mundt, Egbert
对2006年2月暴发期间获得的德国野鸟H5 N1高致病性禽流感病毒索引分离株A/Cygnus cygnus/德国/R65/2006和猫H5 N1分离株A/cat/德国/R606/2006的所有8个片段的全长序列进行分析，发现这两个序列之间具有非常高的相似性。观察到PA基因（编码参与病毒RNA复制的蛋白质）中的一个氨基酸取代和血凝素（HA）蛋白中的一个氨基酸取代。系统发育;对HA和神经氨酸酶核苷酸序列的分析表明，来自位于俄罗斯南部的阿斯特拉罕地区的禽流感H5 N1分离株是最近的亲属。与其他“青海样”H5 N1病毒相比，可以排除重配事件。此外，发现源自德国一次家禽暴发的H5 N1分离株与当时在野鸟种群中流行的H5 N1病毒密切相关。
Analysis of the full-length sequences of all eight segments of the German wild-bird H5N1 highly pathogenic avian influenza virus index isolate, A/Cygnus cygnus/Germany/R65/2006, and an H5N1 isolate from a cat (A/cat/Germany/R606/2006) obtained during an outbreak in February 2006 revealed a very high similarity between these two sequences. One amino acid substitution in the PA gene, encoding a protein involved in virus RNA replication, and one amino acid substitution in the haemagglutinin (HA) protein were observed. Phylogenetic; analyses of the HA and neuraminidase nucleotide sequences showed that avian influenza H5N1 isolates from the Astrakhan region located in southern Russia were the closest relatives. Reassortment events could be excluded in comparison with other 'Qinghai-like' H5N1 viruses. In addition, an H5N1 isolate originating from a single outbreak in poultry in Germany was found to be related closely to the H5N1 viruses circulating at that time in the wild-bird population.