An assessment of methods for aligning two-dimensional microscope sections to create image volumes
An assessment of methods for aligning two-dimensional microscope sections to create image volumes
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DOI：
10.1016/j.jneumeth.2008.01.012
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发表时间：
2008-05-30
影响因子：
3
通讯作者：
Reutens, D.
中科院分区：
文献类型：
--
作者：
Beare, R.;Richards, K.;Reutens, D.
本研究评估了五种不同的方法，用于对齐小鼠大脑Nissl染色切片的显微镜图像，以形成三维图像体积。研究了利用来自未切片组织的图像内容和信息的方法。使用具有已知物理特性的基准点估计重建的准确性，表明利用组织内容的方法产生失真的图像体积，而使用人工基准点的方法产生最准确和无偏的对准。与体积重建方法有关的方法学问题进行了讨论，并建议尽可能使用未切片组织的信息。(C)2008 Elsevier B.V.保留所有权利。
This study assessed five different methods for aligning microscope images of Nissl-stained sections of mouse brain to form three-dimensional image volumes. Methods exploiting both image content and information from un-sectioned tissue were investigated. The accuracy of reconstruction was estimated using fiducials with known physical properties, demonstrating that methods exploiting tissue content produced distorted image volumes while a method using artificial fiducials produced the most accurate and unbiased alignment. Methodological issues relating to methods of volume reconstruction are discussed and it is recommended that methods using information from un-sectioned tissue be used wherever possible. (C) 2008 Elsevier B.V. All rights reserved.