Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae)
Isolation, characterization, cloning and expression of an alpha-neurotoxin from the venom of the Mexican coral snake Micrurus laticollaris (Squamata: Elapidae)
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DOI：
10.1016/j.toxicon.2013.02.006
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发表时间：
2013-05-01
期刊：
影响因子：
2.8
通讯作者：
Olvera-Rodriguez, Alejandro
中科院分区：
文献类型：
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作者：
Carbajal-Saucedo, Alejandro;Lopez-Vera, Estuardo;Olvera-Rodriguez, Alejandro
本文报道了一个新的短链α-神经毒性蛋白家族成员，该家族成员来自墨西哥巨口。该蛋白命名为MlatA 1，具有61个氨基酸和8个保守的半胱氨酸残基，与其他完全测序的Micrurus毒素共享30-91%的序列同一性。MlatA 1（LD（50）i. v.= 0.064 mg/kg）以剂量依赖性方式拮抗胎儿和成人烟碱乙酰胆碱受体（nAChR）以及α-7神经元nAChR。特异性兔抗Mlat血清（滴度高于18，000）则不会。尽管它们对这种毒素显示出任何保护能力，但它能够识别12种Micrurus毒液中的6种中的蛋白质条带，这表明北美珊瑚蛇物种中存在两种不同的α-神经毒素抗原组。MlatA 1基因被克隆并用于产生重组毒素（rMlatA 1），该重组毒素被兔抗天然毒素识别，但毒性活性被耗尽。(C)2013由Elsevier Ltd.出版
A new member of short chain a-neurotoxic protein family from venom of the Mexican coral snake, Micrurus laticollaris, was characterized. This protein, named MlatA1, possesses 61 amino acids with 8 conserved cysteine residues, sharing 30-91% sequence identity with other fully sequenced Micrurus toxins. MlatA1 (LD(50)i.v. = 0.064 mg/kg) antagonizes with both fetal and adult nicotinic acetylcholine receptor (nAChR) as well as alpha-7 neuronal nAChR in a dose-dependent way. Specific rabbit anti-Mlat serum (titer higher than 18,000) does not. show any protective ability against this toxin, nevertheless it was able to recognize protein bands in six out of twelve Micrurus venoms showing the existence of two distinct antigenic groups for alpha-neurotoxins in North American coral snakes species. The MlatA1 gene was cloned and used to produce recombinant toxin (rMlatA1) that was recognized by rabbit anti-native toxin but was depleted of toxic activity. (C) 2013 Published by Elsevier Ltd.