Immobilization of quaternary ammonium based antibacterial monomer onto dentin substrate by non-thermal atmospheric plasma.
Immobilization of quaternary ammonium based antibacterial monomer onto dentin substrate by non-thermal atmospheric plasma.
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DOI：
10.4012/dmj.2018-267
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发表时间：
2019-08
影响因子：
2.5
通讯作者：
Qi Liu;Buling Wu;Qingsong Yu;Yong Wang
中科院分区：
文献类型：
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作者：
Qi Liu;Buling Wu;Qingsong Yu;Yong Wang
采用非热常压等离子体（NTAP）刷将甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）固定在牙本质粘接基质上，研究其对变形链球菌的抗菌活性。从人牙本质中产生具有几微米脱矿层的粘结基底。在等离子体暴露或不暴露的情况下，将DMAHDM施加到脱矿质层上。扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱，以验证DMAHDM固定/接枝到基板上。通过使用菌落形成单位（CFU）和共聚焦扫描激光显微镜来评估所得底物的抗菌活性。并探讨了唾液膜处理和老化过程对上述基片的影响。SEM/FTIR结果表明，NTAP可以诱导DMAHDM固定到牙本质基质上，这进一步通过定量FTIR分析得到验证。与未经等离子体处理的基质相比，经等离子体处理的基质具有更强的抑制作用，CFU低4 log，受唾液或老化的影响最小。DMAHDM固定化牙本质基质显示出有效和持久的抗菌特性。
Use of non-thermal atmospheric plasma (NTAP) brush on immobilization of dimethylaminohexadecyl methacrylate (DMAHDM) onto dentin bonding substrate, and resulting antibacterial activity against Streptococcus mutans were investigated. A bonding substrate with several-micron-demineralized layer was created from human dentin. DMAHDM was applied onto the demineralized layer with or without plasma exposure. Scanning electron microscopy (SEM) and Fourier transformed infrared (FTIR) spectroscopy were employed to verify immobilization/grafting of DMAHDM onto the substrate. Antibacterial activity of the resulting substrate was assessed by using colony-forming unit (CFU) and confocal scanning laser microscopy. Effects of saliva pellicle treatment and aging process on the above substrate were also evaluated. The SEM/FTIR results demonstrated that NTAP could induce DMAHDM immobilization onto dentin substrate, which was further verified via quantitative FTIR analysis. Comparing with non-plasma-treated, the plasmatreated substrate, with CFU 4 log lower, exhibited much stronger inhibitory effects, which were minimally affected by saliva or aging. The DMAHDM-immobilized dentin substrate showed effective and sustained antibacterial characteristics.