ENDOCYTOSIS OF ACETYLATED LOW-DENSITY LIPOPROTEIN, ENDOTHELIAL CELL-MODIFIED LOW-DENSITY LIPOPROTEIN, AND FORMALDEHYDE-TREATED SERUM-ALBUMIN BY RAT-LIVER ENDOTHELIAL-CELLS - EVIDENCE OF UPTAKE VIA A COMMON RECEPTOR
ENDOCYTOSIS OF ACETYLATED LOW-DENSITY LIPOPROTEIN, ENDOTHELIAL CELL-MODIFIED LOW-DENSITY LIPOPROTEIN, AND FORMALDEHYDE-TREATED SERUM-ALBUMIN BY RAT-LIVER ENDOTHELIAL-CELLS - EVIDENCE OF UPTAKE VIA A COMMON RECEPTOR
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DOI：
10.3109/00365528708996474
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发表时间：
1987-12-01
影响因子：
1.9
通讯作者：
BERG, T
中科院分区：
文献类型：
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作者：
ESKILD, W;HENRIKSEN, T;BERG, T
甲醛处理的血清白蛋白 (FSA) 和乙酰化低密度脂蛋白 (Ac-LDL) 在体内和体外被肝窦内皮细胞吸收。目前尚不清楚这两种配体是否被清道夫受体去除。我们研究了增加未标记 FSA、Ac-LDL 和内皮细胞修饰 LDL (Ec-LDL) 浓度对痕量这些 125I 标记配体内吞作用的影响。 FSA 强烈抑制 125I-Ac-LDL 和 125I-Ec-LDL 的摄取。同样，Ac-LDL 和 Ec-LDL 也能有效减少 125I-FSA 的摄取。我们的数据表明，这些修饰的 LDL 和 FSA 通过肝内皮细胞上的相同受体结合并内化。
Formaldehyde-treated serum albumin (FSA) and acetylated low-density lipoprotein (Ac-LDL) are taken up in vivo and in vitro by the sinusoidal endothelial cells of the liver. It is not known whether both these ligands are removed by the scavenger receptor. We have studied the effect of increasing concentrations of unlabeled FSA, Ac-LDL, and endothelial cell-modified LDL (Ec-LDL) on the endocytosis of trace amounts of these ligands labeled with 125I. Uptake of 125I-Ac-LDL and 125I-Ec-LDL was strongly inhibited by FSA. Likewise, Ac-LDL and Ec-LDL reduced the uptake of 125I-FSA effectively. Our data indicate that these modified LDLs and FSA are bound to and internalized via the same receptor on liver endothelial cells.