Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells
Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells
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DOI：
10.1016/j.brainres.2006.09.066
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发表时间：
2006-12-06
期刊：
影响因子：
2.9
通讯作者：
Wada, Akihiko
中科院分区：
文献类型：
--
作者：
Kobayashi, Hideyuki;Yokoo, Hiroki;Wada, Akihiko
大脑中的水平衡对大脑功能至关重要。我们研究了糖皮质激素对GP 8永生化的大鼠脑微血管内皮细胞水通道蛋白（AQP）1表达的调控。地塞米松处理后AQP 1蛋白水平呈时间和浓度依赖性升高。地塞米松对AQP 1蛋白表达的上调与AQP 1 mRNA水平的增加有关，但对AQP 1 mRNA降解率无影响。AQP 1被浓缩在洗涤剂不溶性组分中的细胞用或不用地塞米松处理，这表明AQP 1的功能/贩运可能是通过与脂筏的相互作用来调节。由于糖皮质激素治疗在脑水肿的治疗中具有众所周知的有益效果，地塞米松对AQP 1的诱导提高了AQP 1在改善脑水肿中起作用的可能性。(c)2006 Elsevier B. V.保留所有权利。
Water homeostasis in the brain is essential for brain function. We have studied how aquaporin (AQP) 1 expression in GP8 immortalized rat brain microvascular endothelial cells is regulated by glucocorticoid. AQP1 protein level was raised by dexamethasone treatment in a time- and concentration-dependent manner. The up-regulation of AQP1 protein by dexamethasone was associated with an increase of AQP1 mRNA level, with no change in the degradation rate of AQP1 mRNA. AQP1 was concentrated in detergent-insoluble fractions in the cells treated with or without dexamethasone, suggesting that function/trafficking of AQP1 may be regulated via the interaction with lipid rafts. Since glucocorticoid therapy has well known beneficial effects in the treatment of brain edema, the induction of AQP1 by dexamethasone raises a possibility that AQP1 plays a role in ameliorating brain edema. (c) 2006 Elsevier B.V. All rights reserved.