Real-time nanoscopy by using blinking enhanced quantum dots.
Real-time nanoscopy by using blinking enhanced quantum dots.
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DOI：
10.1016/j.bpj.2010.07.036
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发表时间：
2010-10
影响因子：
3.4
通讯作者：
Tomonobu M. Watanabe;S. Fukui;T. Jin;F. Fujii;T. Yanagida
中科院分区：
文献类型：
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作者：
Tomonobu M. Watanabe;S. Fukui;T. Jin;F. Fujii;T. Yanagida
超分辨光学显微镜（纳米显微镜）是目前许多生物领域的研究热点。利用荧光时间涨落的高阶累积量的超分辨光学涨落成像技术，由于不需要复杂的光学系统和光源，是一种很好的纳米成像方法。然而，它确实需要一个不切实际的图像数量的实时观察。在这里，我们通过改进超分辨光学涨落成像和增强量子点的涨落来实现实时纳米显微镜。我们开发的量子点比商业上可用的量子点具有更高的闪烁。闪烁的波动提高了分辨率时，使用每个像素的方差计算，而不是累积计算。这使我们能够获得90纳米和80毫秒的时空分辨率的显微图像，通过使用传统的荧光显微镜没有任何光学或设备。
Superresolution optical microscopy (nanoscopy) is of current interest in many biological fields. Superresolution optical fluctuation imaging, which utilizes higher-order cumulant of fluorescence temporal fluctuations, is an excellent method for nanoscopy, as it requires neither complicated optics nor illuminations. However, it does need an impractical number of images for real-time observation. Here, we achieved real-time nanoscopy by modifying superresolution optical fluctuation imaging and enhancing the fluctuation of quantum dots. Our developed quantum dots have higher blinking than commercially available ones. The fluctuation of the blinking improved the resolution when using a variance calculation for each pixel instead of a cumulant calculation. This enabled us to obtain microscopic images with 90-nm and 80-ms spatial-temporal resolution by using a conventional fluorescence microscope without any optics or devices.