Interaction of brain synaptic vesicles induced by endogenous Ca2+ -dependent phospholipase A2.
Interaction of brain synaptic vesicles induced by endogenous Ca2+ -dependent phospholipase A2.
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内源性 Ca2 依赖性磷脂酶 A2 诱导的脑突触小泡的相互作用。
DOI：
10.1126/science.6278597
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Puszkin,S
中科院分区：
文献类型：
--
作者：
Moskowitz,N;Schook,W;Puszkin,S
脑突触小泡的内源性磷脂酶A2活性依赖于钙离子，前列腺素F2α、钙调素、腺苷3‘，5’-单磷酸和三磷酸腺苷均能增强其活性，但在无钙调素或有钙调素存在的情况下，前列腺素E2可抑制其活性。光散射测量表明，酶活性的刺激与囊泡-囊泡聚集的诱导有关。这些化合物对内源性突触囊泡磷脂酶A2活性的影响可能暗示了它们所谓的神经调节作用的共同终点，并提示突触囊泡磷脂酶A2可能在突触前神经传递中发挥核心作用。
Endogenous phospholipase A2activity of brain synaptic vesicles was Ca2+-dependent and was increased by prostaglandinF2α, calmodulin, adenosine 3′,5′ -monophosphate, and adenosine triphosphate, whereas the activity was inhibited by prostaglandin E2in the absence or presence of calmodulin. Light-scattering measurements demonstrated that stimulation of the enzyme's activity correlated with the induction of vesicle-vesicle aggregation. The effects of these compounds on endogenous synaptic vesicle phospholipase A2activity may imply a common end point of their purported neuromodulatory actions, and indicate that synaptic vesicle phospholipase A2may play a central role in presynaptic neurotransmission.