Pyruvate Metabolism in Saccharomyces cerevisiae
Pyruvate Metabolism in Saccharomyces cerevisiae
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DOI：
10.1002/(sici)1097-0061(199612)12:16
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发表时间：
1996-12
期刊：
影响因子：
2.6
通讯作者：
J. Pronk;H. Yde Steensma;J. P. van Dijken
中科院分区：
文献类型：
--
作者：
J. Pronk;H. Yde Steensma;J. P. van Dijken
在酵母中，丙酮酸位于同化和异化反应的主要连接点以及糖的呼吸异化和酒精发酵之间的分支点。本文综述了酿酒酵母中丙酮酸分支点发生的三个关键反应的酶学、生理功能和调控：（i）由丙酮酸脱氢酶复合物催化的丙酮酸直接氧化脱羧为乙酰辅酶A，（ii）由丙酮酸脱羧酶催化的丙酮酸脱羧为乙醛，和（iii）丙酮酸回补羧化为草酰乙酸，由丙酮酸羧化酶催化。特别注意的生理研究，致力于S。其中编码这些关键酶的结构基因已通过基因破坏失活。
In yeasts, pyruvate is located at a major junction of assimilatory and dissimilatory reactions as well as at the branch‐point between respiratory dissimilation of sugars and alcoholic fermentation. This review deals with the enzymology, physiological function and regulation of three key reactions occurring at the pyruvate branch‐point in the yeast Saccharomyces cerevisiae: (i) the direct oxidative decarboxylation of pyruvate to acetyl‐CoA, catalysed by the pyruvate dehydrogenase complex, (ii) decarboxylation of pyruvate to acetaldehyde, catalysed by pyruvate decarboxylase, and (iii) the anaplerotic carboxylation of pyruvate to oxaloacetate, catalysed by pyruvate carboxylase. Special attention is devoted to physiological studies on S. cerevisiae strains in which structural genes encoding these key enzymes have been inactivated by gene disruption.