Markers for assessment of nodal metastasis in laryngeal carcinoma.
Markers for assessment of nodal metastasis in laryngeal carcinoma.
复制标题
评估喉癌淋巴结转移的标志物。
DOI：
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复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. V. Krieken
中科院分区：
文献类型：
--
作者：
R. Takes;R. B. Jong;E. Schuuring;J. Hermans;André A. Vis;S. Litvinov;J. V. Krieken
背景
区域转移是影响头颈部鳞状细胞癌患者治疗和预后的重要因素。虽然近年来影像学技术有所改进，但仍无法检测到小的转移性沉积物。转移主要取决于原发肿瘤的性质及其与周围结构的相互作用。
目的
根据原发肿瘤的特征，确定预测转移存在的标志物。
设计
组织学、免疫组化和分子生物学分析结果与临床和组织病理学数据的相关性。
材料和方法
本文对31例喉癌的组织学特征和生物学标志物进行了研究。根据其在转移过程中的假定作用选择以下标志物，并使用免疫组织化学和/或Southern印迹技术进行研究：增殖细胞核抗原（PCNA）、p53、视网膜母细胞瘤肿瘤抑制基因（Rb）、myc、bcl-2（细胞凋亡抑制剂），表皮生长因子（EGF），EGF受体（EGFR），neu，nm 23（也称为NME 1，推定的转移抑制因子）、桥粒斑蛋白、神经元细胞粘附分子（N-CAM）、上皮细胞粘附分子（Ep-CAM）、E-钙粘蛋白、细胞周期蛋白D1（CCND 1）和EMS 1。
结果
肿瘤周围炎性反应（P = 0.07）、嗜酸性粒细胞浸润（P = 0.16）、Rb免疫染色阳性（P = 0.02）、Ep-CAM免疫染色阴性（P = 0.13）、CCND 1和EMS 1扩增（P = 0.05）与淋巴结转移相关。炎症反应、嗜酸性粒细胞浸润、Rb和Ep-CAM染色的组合导致评估淋巴结转移的上级准确性。
结论
这些结果表明，仅通过研究原发肿瘤的特征来预测和排除淋巴结转移是可能的。当这些结果在更大的系列中得到证实时，生物标志物可能是对临床决策具有重大影响的强大诊断工具。
BACKGROUND
Regional metastasis is an important factor in the treatment and prognosis of patients with head and neck squamous cell carcinoma. Although in recent years imaging techniques have improved, it is still impossible to detect small metastatic deposits. Metastasis is mainly determined by properties of the primary tumor and its interaction with surrounding structures.
OBJECTIVE
To identify markers that predict the presence of metastasis based on the features of the primary tumor.
DESIGN
Correlation of the results of histological, immunohistochemical, and molecular biological analysis with clinical and histopathological data.
MATERIALS AND METHODS
Several histological features and biological markers were examined in 31 laryngeal carcinomas. The following markers were selected on their putative role in the process of metastasis and were studied using immunohistochemical and/or Southern blot techniques: proliferating cell nuclear antigen (PCNA), p53, retinoblastoma tumor-suppressor gene (Rb), myc, bcl-2 (inhibitor of apoptosis), epidermal growth factor (EGF), EGF-receptor (EGFR), neu, nm23 (also known as NME1, putative metastasis suppressor), desmoplakin, neuron cell-adhesion molecule (N-CAM), epithelial cell-adhesion molecule (Ep-CAM), E-cadherin, cyclin D1 (CCND1), and EMS1.
RESULTS
The presence of an inflammatory reaction surrounding the tumor (P = .07), eosinophilic infiltration (P = .16), positive immunostaining for Rb (P = .02), negative immunostaining for Ep-CAM (P = .13), and amplification of CCND1 and EMS1 (P = .05) correlated with nodal metastasis. The combination of an inflammatory reaction, eosinophilic infiltration, and staining for Rb and Ep-CAM resulted in a superior accuracy in assessing nodal metastasis.
CONCLUSIONS
These results indicate that it is possible to predict and exclude lymph node metastasis by studying the features of the primary tumor only. When these results are confirmed in a larger series, biological markers may be powerful diagnostic tools with great impact on clinical decision making.
头颈鳞状细胞癌中染色体 11Q13 扩增。
DOI：
10.1001/archotol.1993.01880230084013
发表时间：
1993
期刊：
Archives of otolaryngology--head & neck surgery
影响因子：
--
作者：
Williams,ME;Gaffey,MJ;Weiss,LM;Wilczynski,SP;Schuuring,E;Levine,PA
通讯作者：
Levine,PA
一项关于根治性和改良颈清扫术导致肩部残疾的前瞻性研究。
DOI：
10.1002/hed.2890080408
发表时间：
1986
期刊：
Head & neck surgery
影响因子：
--
作者：
Remmler,D;Byers,R;Scheetz,J;Shell,B;White,G;Zimmerman,S;Goepfert,H
通讯作者：
Goepfert,H