Expression of chick Tbx-2, Tbx-3, and Tbx-5 genes during early heart development: evidence for BMP2 induction of Tbx2.
Expression of chick Tbx-2, Tbx-3, and Tbx-5 genes during early heart development: evidence for BMP2 induction of Tbx2.
复制标题
DOI：
10.1006/dbio.2000.9927
复制
发表时间：
2000-12
影响因子：
2.7
通讯作者：
Miho Yamada;J. Revelli;G. Eichele;Matt Barron;R. Schwartz
中科院分区：
文献类型：
--
作者：
Miho Yamada;J. Revelli;G. Eichele;Matt Barron;R. Schwartz
分析了Tbx2、-3和-5基因在鸡胚心脏发育过程中的表达规律。这三个cTbx基因的转录本在早期心脏新月中被检测到重叠模式。cTbx2和cTbx3的表达模式与bmp2的表达模式紧密重叠。cTbx5与cTbx2、bmp2的表达在心管发育和折叠过程中发生分化。相比之下，在心脏发育的所有阶段和胚胎后期，cTbx2的表达与bmp2和bmp4的表达显著重叠，提示Tbx2在分离中起特殊作用。ctbx2和cTbx3基因与bmp2转录物的共表达提高了这些cTbx基因可能是bmp2下游靶点的可能性。应用bmp2选择性诱导cTbx2和cTbx3在非心源性胚胎组织中的表达，bmp拮抗剂Noggin下调cTbx2基因活性。此外，小鼠mmtx2的出现在bmp2缺失的小鼠胚胎中被阻断。cTbx2和cTbx3基因活性在较低程度上似乎受早期心脏发生过程中bmp的指导。
Expression patterns of Tbx2, -3, and -5 genes were analyzed during chick embryonic heart development. Transcripts of these three cTbx genes were detected in overlapping patterns in the early cardiac crescent. cTbx2 and cTbx3 expression patterns closely overlapped with that of bmp2. cTbx5 expression diverged from cTbx2 and bmp2 during the elaboration and folding of the heart tube. In comparison, cTbx2 expression overlapped significantly with that of bmp2 and bmp4 during all stages of heart development and during later embryonic stages, suggestive of a specialized role for Tbx2 in septation. Coexpression of cTbx 2 and cTbx3 genes with bmp2 transcripts raised the possibility that these cTbx genes might be downstream bmp2 targets. Application of bmp2 selectively induced cTbx2 and cTbx3 expression in noncardiogenic embryonic tissue, and the bmp antagonist Noggin down-regulated cTbx2 gene activity. Moreover, the appearance of murine mTbx2 was blocked in bmp2 null mouse embryos. cTbx2 and to a lesser extent cTbx3 gene activity appears to be directed by BMPs during early cardiogenesis.