Increased expression of the transcription factor E2F1 during dopamine-evoked, caspase-3-mediated apoptosis in rat cortical neurons
Increased expression of the transcription factor E2F1 during dopamine-evoked, caspase-3-mediated apoptosis in rat cortical neurons
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DOI：
10.1016/s0304-3940(01)01909-7
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发表时间：
2001-06-29
影响因子：
2.5
通讯作者：
MacManus, JP
中科院分区：
文献类型：
--
作者：
Hou, ST;Cowan, E;MacManus, JP
在多巴胺(DA)或6-羟基多巴胺(OHDA)诱导的细胞凋亡中，转录因子E2F1的mRNA和蛋白水平在大鼠皮质神经元中升高。用抗E2F1和NeuN抗体进行双荧光免疫细胞化学染色，检测到神经元胞核中E2F1蛋白表达增加。DA和6-OHDA诱导caspase-3介导的皮质神经元凋亡，这种作用可被加入抗氧化剂或caspase-3抑制剂所减弱。抗氧化剂可阻止DA诱导的神经细胞凋亡，并可抑制E2F1表达的增加。这些结果表明，转录因子E2F1的表达增加可能是DA诱导的神经元凋亡过程中的死亡信号。(C)2001年，爱思唯尔科学爱尔兰有限公司出版。
The transcription factor E2F1 mRNA and protein levels increased in rat cortical neurons in response to dopamine (DA)or 6-hydroxydopamine (OHDA)-evoked apoptosis. Increased E2F1 protein was detected in the nucleus of neurons by double fluorescent immunocytochemistry using antibodies to E2F1 and NeuN. DA and 6-OHDA induced caspase-3-mediated apoptosis of cortical neurons which was attenuated by the addition of antioxidants or caspase-3 inhibitors to the cultures. Antioxidants prevented DA-evoked neuronal apoptosis, and also attenuated the increase in E2F1 expression. These findings suggest that increased expression of the transcription factor E2F1 may serve as a death signal during DA-evoked neuronal apoptosis. (C) 2001 Published by Elsevier Science Ireland Ltd.