CONTROL OF DOUBLESEX ALTERNATIVE SPLICING BY TRANSFORMER AND TRANSFORMER-2 IN DROSOPHILA
CONTROL OF DOUBLESEX ALTERNATIVE SPLICING BY TRANSFORMER AND TRANSFORMER-2 IN DROSOPHILA
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DOI：
10.1126/science.1902987
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发表时间：
1991-05-10
期刊：
影响因子：
56.9
通讯作者：
SHIMURA, Y
中科院分区：
文献类型：
--
作者：
HOSHIJIMA, K;INOUE, K;SHIMURA, Y
双性（dsx）前体信使RNA（pre-mRNA）的性别特异性替代加工调节黑腹果蝇的体细胞性分化。 共转染分析，其中dsx基因和女性特异性的Transformer（tra）和Transformer-2（tra-2）互补DNA在果蝇KC细胞中表达显示，女性特异性剪接的dsx转录正调控的tra和tra-2基因的产品。 此外，dsx的突变体结构的分析表明，女性特异性外显子序列的一部分所需的调控dsx前信使RNA剪接。
Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster. Cotransfection analyses in which the dsx gene and the female-specific transformer (tra) and transformer-2 (tra-2) complementary DNAs were expressed in Drosophila Kc cells revealed that female-specific splicing of the dsx transcript was positively regulated by the products of the tra and tra-2 genes. Furthermore, analyses of mutant constructs of dsx showed that a portion of the female-specific exon sequence was required for regulation of dsx pre-messenger RNA splicing.