Horseradish peroxidase: the analyst's friend.
Horseradish peroxidase: the analyst's friend.
复制标题
DOI：
--
复制
发表时间：
1994
影响因子：
6.4
通讯作者：
O. Ryan;M. Smyth;C. Ó. Fágáin
中科院分区：
文献类型：
--
作者：
O. Ryan;M. Smyth;C. Ó. Fágáin
HRP是一种含钙、广泛糖基化、稳定的血红蛋白，具有四个二硫键，分子量为44 kDa。它是一种氧化还原酶，可以使用多种氢供体来还原过氧化氢。这就产生了一系列HRP活性的比色、荧光、荧光和电化学测定。HRP作为指示剂广泛应用于免疫分析、非同位素DNA探针、细胞化学、双酶系统和生物传感器。这是由于其稳定性、高催化速率、易于与其他分子缀合以及活性测定的广泛选择。
HRP is a calcium-containing, extensively glycosylated, stable haemoprotein possessing four disulphide bridges and weighing 44 kDa. It is an oxidoreductase which can use a wide variety of hydrogen donors to reduce hydrogen peroxide. This gives rise to a range of colorimetric, fluorimetric, chemiluminescent and electrochemical assays for HRP activity. HRP is very widely used as an indicator in immunoassays, non-isotopic DNA probes, cytochemistry, bi-enzyme systems and biosensors. This is owing to its stability, high catalytic rates, ease of conjugation to other molecules and wide choice of assays of activity.