NUCLEOTIDE-SEQUENCE OF 42 KBP OF VACCINIA VIRUS-STRAIN WR FROM NEAR THE RIGHT INVERTED TERMINAL REPEAT
NUCLEOTIDE-SEQUENCE OF 42 KBP OF VACCINIA VIRUS-STRAIN WR FROM NEAR THE RIGHT INVERTED TERMINAL REPEAT
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DOI：
10.1099/0022-1317-72-6-1349
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发表时间：
1991-06-01
影响因子：
3.8
通讯作者：
HOWARD, ST
中科院分区：
文献类型：
--
作者：
SMITH, GL;CHAN, YS;HOWARD, ST
本文报道了痘苗病毒WR株42090 bp的核苷酸序列。该序列包括SalI L、F、G和I片段，并在HindIII A片段的中心附近开始，向右延伸至基因组末端，在反向末端重复序列（ITR）内部约0.5 kb处结束。该区域的翻译已鉴定出65个长度大于65个氨基酸的开放阅读框（ORF）。其中51个与其他较大的ORF没有广泛重叠，已经进行了进一步的分析;其他14个被称为次要ORF。在最右边的28.7 kb中，除了一个例外，基因被转录到基因组末端，类似于病毒基因组左端的基因排列。在该区域内部，基因在任一DNA链外表达，但仍主要向右表达。ORF紧密排列，只有少数大于250 bp的基因间非编码区。蛋白质序列比较已经建立了显着的高数目的同源性与现有的蛋白质数据库中的条目。其中，DNA连接酶，胸苷酸激酶，两个丝氨酸-苏氨酸蛋白激酶，两个丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂），两个白细胞介素-1受体同源物和一个不连续的ORF相关的肿瘤坏死因子受体已被报道。其他同源物包括凝集素、profilin、3-β-羟基类固醇脱氢酶、超氧化物歧化酶、鸟苷酸激酶、锚蛋白和补体因子H。此外，存在许多具有膜结合、分泌或糖蛋白的预测性质的多肽。在这里和其他地方描述了12个基因家族。来自病毒基因组右端和左端的基因之间存在相当大的相似性，这可能是由末端转座事件引起的。注意到与牛痘病毒株哥本哈根序列的相应区域的几个差异。在靠近右末端的序列完全分叉，并且在其内部存在位于五核苷酸或六核苷酸直接重复内的短序列（8至10个核苷酸）缺失的多个实例。
The nucleotide sequence of 42090 bp of vaccinia virus strain WR is presented. The sequence includes the SalI L.F,G and I fragments and starts near the centre of the HindIII A fragment and extends rightwards towards the genomic terminus, finishing approximately 0.5 kb internal of the inverted terminal repeat (ITR). Translation of this region has identified 65 open reading frames (ORFs) of greater than 65 amino acids in length. Fifty-one of these which do not extensively overlap other larger ORFs have been subjected to further analysis; the other 14 are termed minor ORFs. In the rightmost 28.7 kb, the genes are, with one exception, transcribed towards the genomic terminus, similar to the arrangement of genes at the left end of the virus genome. Internal of this region the genes are expressed off either DNA strand but still predominately rightwards. ORFs are tightly packed with few intergenic non-coding regions of greater than 250 bp. Protein sequence comparisons have established a remarkably high number of homologies with entries in existing protein databases. Of these, DNA ligase, thymidylate kinase, two serine-threonine protein kinases, two serine proteinase inhibitors (serpins), two interleukin-1 receptor homologues and a discontinuous ORF related to tumour necrosis factor receptor have been reported. Other homologies include lectins, profilin, 3-beta-hydroxy steroid dehydrogenase, superoxide dismutase, guanylate kinase, ankyrin and complement factor H. In addition, there are a number of polypeptides with predicted properties of membrane-associated, secretory or glyco-proteins. Twelve gene families are described here and elsewhere. There is considerable similarity between genes from the right and left end of the virus genome that may have arisen by terminal transposition events. Several differences from the corresponding region of vaccinia virus strain Copenhagen sequence are noted. Near the right terminus the sequences diverge completely, and internal of this there are multiple examples of deletion of short sequences (eight to 10 nucleotides) that lie within penta- or hexanucleotide direct repeats.