Temporal and spatial variations of lipid droplets during adipocyte division and differentiation
Temporal and spatial variations of lipid droplets during adipocyte division and differentiation
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DOI：
10.1194/jlr.m600155-jlr200
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发表时间：
2007-01-01
影响因子：
6.5
通讯作者：
Gohara, Kazutoshi
中科院分区：
文献类型：
--
作者：
Nagayama, Masafumi;Uchida, Tsutomu;Gohara, Kazutoshi
通过对大鼠肠系膜脂肪组织原代基质血管细胞（SVCs）的延时成像，我们揭示了脂肪细胞分化过程中单个SVCs中脂滴（ld）的时空变化。在分化早期，在核周区域出现了许多小ld（直径几微米）；随后，一些小点生长到直径超过10mm并占据细胞质。我们建立了一种活体脂肪细胞ld荧光染色的方法。在高倍镜下对染色细胞的延时观察显示，在从板足向核周区域移动的过程中，新生的lld（直径为几个100 nm）逐渐发育成小的lld。我们还发现脂肪细胞能够分裂，并且它们在两个子细胞之间均匀地分配ld。免疫荧光观察ld相关蛋白发现，即使在ld被涂覆后，SVCs的这种细胞分裂也会发生，这表明脂肪细胞分化过程中的“最终”细胞分裂发生的时间明显晚于3T3-L1细胞。我们的延时观察提供了SVCs在脂肪细胞分裂和分化过程中形态变化的详细描述。
By capturing time-lapse images of primary stromal-vascular cells (SVCs) derived from rat mesenteric adipose tissue, we revealed temporal and spatial variations of lipid droplets (LDs) in individual SVCs during adipocyte differentiation. Numerous small LDs (a few micrometers in diameter) appeared in the perinuclear region at an early stage of differentiation; subsequently, several LDs grew to more than 10 mm in diameter and occupied the cytoplasm. We have developed a method for the fluorescence staining of LDs in living adipocytes. Time-lapse observation of the stained cells at higher magnification showed that nascent LDs (several 100 nm in diameter) grew into small LDs while moving from lamellipodia to the perinuclear region. We also found that adipocytes are capable of division and that they evenly distribute the LDs between two daughter cells. Immunofluorescence observations of LD-associated proteins revealed that such cell divisions of SVCs occurred even after LDs were coated with perilipin, suggesting that the "final" cell division during adipocyte differentiation occurs considerably later than that characterized in 3T3-L1 cells. Our time-lapse observations have provided a detailed account of the morphological changes that SVCs undergo during adipocyte division and differentiation.